Department of Cariology and Operative Dentistry, Division of Oral Health Science, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Japan.
Department of Cariology and Operative Dentistry, Division of Oral Health Science, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Japan; Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University, Thailand; Research Unit of Biomimetic and Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Thailand.
J Mech Behav Biomed Mater. 2024 Feb;150:106295. doi: 10.1016/j.jmbbm.2023.106295. Epub 2023 Dec 10.
To evaluate the effect of ions released from surface pre-reacted glass-ionomer (S-PRG) filler on collagen morphology, remineralization, and ultimate tensile strength (UTS) of demineralized dentin.
Bovine incisor root dentins were demineralized with EDTA and divided into three treatment groups: 1) water (control); 2) S-PRG filler eluate; 3) 125 ppm sodium fluoride (NaF). After a 3-min treatment, the specimens were stored in simulated body fluid (SBF) for 3 months. Collagen morphology and remineralization were assessed using transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR). Additionally, ultimate tensile strength (UTS) was measured.
TEM and SEM demonstrated that S-PRG induced more effective remineralization compared to NaF, while the control group exhibited faint mineral deposition with collagen degradation. S-PRG displayed the most homogenous mineral deposition in collagen fibrils, along with closure of interfibrillar spaces. Extensive mineral precipitation was observed within dentinal tubules in the S-PRG group. In addition, S-PRG filler eluate demonstrated significantly higher phosphate-to-amide ratio and UTS compared to NaF and control groups (p < 0.05).
Ion released from S-PRG filler positively influenced collagen morphology, remineralization, and ultimate tensile strength of demineralized dentin.
S-PRG filler enhances remineralization and improve the biomechanics of demineralized dentin.
评估表面预反应玻璃离子（S-PRG）填料释放的离子对脱矿牙本质胶原形态、再矿化和极限拉伸强度（UTS）的影响。
用 EDTA 脱矿牛切牙根牙本质，分为三组：1）水（对照）；2）S-PRG 填料浸提液；3）125ppm 氟化钠（NaF）。处理 3 分钟后，将标本在模拟体液（SBF）中储存 3 个月。采用透射电子显微镜（TEM）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）评估胶原形态和再矿化。此外，还测量了极限拉伸强度（UTS）。
TEM 和 SEM 表明，与 NaF 相比，S-PRG 诱导了更有效的再矿化，而对照组显示胶原降解伴有微弱的矿物质沉积。S-PRG 在胶原纤维中显示出最均匀的矿物质沉积，同时也封闭了纤维间的空间。在 S-PRG 组中，牙本质小管内观察到广泛的矿物质沉淀。此外，S-PRG 填料浸提液的磷酸盐/酰胺比和 UTS 明显高于 NaF 和对照组（p<0.05）。
S-PRG 填料释放的离子对脱矿牙本质的胶原形态、再矿化和极限拉伸强度有积极影响。
S-PRG 填料增强了再矿化，并改善了脱矿牙本质的生物力学性能。
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