Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Department of Genetics, Morphology, Orthodontics, and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
J Dent. 2022 Sep;124:104237. doi: 10.1016/j.jdent.2022.104237. Epub 2022 Jul 18.
To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation.
Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX - inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%).
S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control.
Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days.
S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces.
评估释离子填充剂（S-PRG）浸提液对牙本质胶原结合基质金属蛋白酶（MMPs）和牙本质基质降解的抑制活性。
用人磨牙制备完全脱矿的牙本质梁（5×2×0.5mm），以产生牙本质基质标本。测量干重，并用比色法（Sensolyte）测定初始总 MMP 活性，将梁分为四组（n=10/组）：1）水 1 分钟（阴性对照）；2）2%葡萄糖酸氯己定（CHX-抑制剂对照）1 分钟；3）S-PRG 浸提液 1 分钟；4）S-PRG 浸提液 30 分钟。处理后重新评估总 MMP 活性。标本在 37°C 的模拟体液（SBF）中储存长达 21 天。每周重新评估干重。第 7 天，分析牙本质基质降解情况，检测 SBF 中胶原片段（CF；Sirius Red）和羟脯氨酸（Hyp）的存在。采用方差分析/Tukey、配对 t 检验和 RM-ANOVA/Sidak（α=5%）进行统计分析。
S-PRG 浸提液暴露 1 分钟和 30 分钟可降低 MMP 活性（p<0.0001）。S-PRG 暴露 30 分钟时，MMP 活性抑制与 CHX 相当（p=0.061）。S-PRG 和 CHX 减少 CF（p≤0.007）和 Hyp（p<0.046）的释放。储存 21 天后，无论暴露时间如何，S-PRG 处理的梁的质量损失均减少（p≤0.017），介于 CHX 和对照组之间。
用 S-PRG 浸提液处理脱矿牙本质 1 分钟或 30 分钟可降低基质结合 MMP 活性，并在 21 天内减少牙本质基质降解。
S-PRG 填充剂可能阻碍牙本质龋/侵蚀性病变的进展，并增强牙本质粘结界面的稳定性。
