Hayashi K, Kagawa K, Awai M, Irino S
Scan Electron Microsc. 1986(Pt 4):1489-99.
Bone marrow aplasia was induced in rats by whole body lethal irradiation (1,000 rads by x-ray), and rats died of irradiation injury within 7 days. Correlative studies at light (LM), transmission (TEM) and scanning electron microscopy (SEM) demonstrated swelling of endothelial and reticular cells and hemorrhage due to detachment of sinus endothelial cells on days 1 and 2. With time, structural recovery occurred without hemopoietic recovery. Reticular cells developed small intracytoplasmic lipid droplets on days 3 and 4. This resulted in fatty aplastic marrow within 7 days. On the other hand, in the marrow of irradiated rats parabiosed with healthy mates by aortic anastomosis, hemopoiesis was initiated by adhesion of nucleated blood cells to fine cytoplasmic pseudopods of fat-stored cells on days 1 and 2 after parabiosis. On days 3 to 5, reticular cells with large lipid droplets and fine pseudopods increased, then hemopoietic foci became clear and extensive. On day 8 after parabiosis, the aplastic bone marrow recovered completely both its structure and hemopoietic activity. Thus, hemopoietic recovery in lethally irradiated marrow begins with recovery of vascular endothelial cells, re-establishment of sinusoidal structure, and morphological and functional recoveries of reticular cells from fat-storage cells by releasing intracytoplasmic lipid droplets. Marrow stromal cells, namely reticular, fat-storage and fibroblastoid cells, share a common cellular origin, and regain their structure and function when fat-storage cells and fibroid cells are placed in contact with hemopoietic precursor cells.
通过全身致死性照射（1000拉德X射线）诱导大鼠发生骨髓再生障碍，大鼠在7天内死于辐射损伤。在第1天和第2天，光镜（LM）、透射电镜（TEM）和扫描电镜（SEM）的相关研究显示内皮细胞和网状细胞肿胀以及由于窦内皮细胞脱离导致的出血。随着时间推移，结构恢复但造血未恢复。在第3天和第4天，网状细胞形成小的胞质内脂滴。这导致在7天内形成脂肪性再生障碍性骨髓。另一方面，在通过主动脉吻合与健康同伴联体的受照射大鼠的骨髓中，联体后第1天和第2天，有核血细胞粘附到储存脂肪细胞的细细胞质伪足上启动了造血。在第3至5天，具有大脂滴和细伪足的网状细胞增加，然后造血灶变得清晰且广泛。联体后第8天，再生障碍性骨髓的结构和造血活性完全恢复。因此，致死性照射骨髓中的造血恢复始于血管内皮细胞的恢复、血窦结构的重建以及网状细胞通过释放胞质内脂滴从储存脂肪细胞进行形态和功能的恢复。骨髓基质细胞，即网状细胞、储存脂肪细胞和成纤维样细胞，具有共同的细胞起源，并且当储存脂肪细胞和纤维样细胞与造血前体细胞接触时恢复其结构和功能。
