Seed T M, Chubb G T, Tolle D V
Scan Electron Microsc. 1981;4:61-72.
Beagles continuously exposed to low daily doses (10 R) of whole-body 60Co gamma-radiation are prone to develop either early occurring aplastic anemia or late occurring myeloproliferative disorders (Seed et al., 1977). In this study, we have examined by a combination of light microscopy and scanning and transmission electron microscopy the sequential changes in the morphology of biopsied rib bone marrow of continuously irradiated dogs that developed either aplastic anemia, myelofibrosis, or myelogenous leukemia. Characteristic modification of key elements of marrow architecture have been observed during preclinical and clinical phases of these hemopathological conditions. The more prominent of these changes include the following. (i) In developing aplastic anemia: severe vascular sinus and parenchymal cord compression, and focally degenerate endosteal surfaces. (ii) In developing myelofibrosis: hyperplasia of endosteal and reticular stomal elements. (iii) In developing leukemia: hypertrophy of reticular and endothelial elements in the initial restructuring of the stromal matrix and the subsequent aberrant hemopoietic repopulation of the initially depleted stromal matrix. These architectural changes during preclinical phases appear to be related to the pathological progression to each of the radiation-induced hemopathological end points.
持续暴露于低剂量（10拉德）全身60钴γ射线的比格犬易于发生早期再生障碍性贫血或晚期骨髓增殖性疾病（Seed等人，1977年）。在本研究中，我们通过光学显微镜、扫描电子显微镜和透射电子显微镜相结合的方法，研究了持续照射后发生再生障碍性贫血、骨髓纤维化或髓性白血病的犬活检肋骨骨髓形态的连续变化。在这些血液病理学疾病的临床前和临床阶段，观察到了骨髓结构关键要素的特征性改变。其中更显著的变化如下。（i） 在发展为再生障碍性贫血的过程中：严重的血管窦和实质索压迫，以及局部变性的骨内膜表面。（ii） 在发展为骨髓纤维化的过程中：骨内膜和网状间质成分增生。（iii） 在发展为白血病的过程中：在基质的初始重组中网状和内皮成分肥大，随后初始耗尽的基质出现异常造血再填充。临床前阶段的这些结构变化似乎与每种辐射诱导的血液病理学终点的病理进展有关。
