Division of Bacteriology and Pathology, Department of Pathology, Institute for Experimental Pathology at Keldur, Keldnavegi 3, 112, Reykjavík, Iceland.
Faculty of Agricultural Sciences, Agricultural University of Iceland, Árleyni 22, 112, Reykjavík, Iceland.
Acta Vet Scand. 2023 Dec 15;65(1):57. doi: 10.1186/s13028-023-00721-x.
Few studies have been conducted on haematological reference intervals (RIs) in Icelandic horses. Reference intervals have been published for Icelandic horses in Austria and a preliminary study in Iceland compared haematological values in riding horses to published RIs for other breeds as well as Icelandic horses abroad. Haematological parameters can vary greatly due to factors such as breed, gender, age, reproductive status, and training, as well as feeding, prior exercise and management method. Icelandic broodmares are kept on pasture under supervision throughout the year, with haylage provided during the winter, and it is therefore of interest to establish haematological reference intervals for pregnant broodmares in Iceland. The purpose of this study was to establish haematological RIs specific to Icelandic broodmares in the first months of pregnancy, kept on pasture. Blood samples from 183 mares, stabilised in EDTA were analysed using IDEXX ProCyte Dx and total protein was analysed in serum samples from 157 of the 183 mares, using IDEXX Catalyst One analyser. The RIs were established using the guidelines of the American Society for Veterinary Clinical Pathology.
The RIs for red blood cell count, haematocrit and haemoglobin were higher in pasture-kept Icelandic mares in early pregnancy, most of which were lactating, than in pregnant mares of other breeds. This was also true for white blood cell count, as well as numbers of monocytes, eosinophils, and basophils, which in some instances might illustrate problems in the automated categorisation of some leukocytes.
As no RIs have been published for other pasture-kept Icelandic horses, future investigations should include other groups of pasture-kept Icelandic horses. Such an analysis might elucidate the effect of breed, management, and pregnancy on haematological values in pasture-kept Icelandic horses.
冰岛马的血液学参考区间(RI)研究较少。已经在奥地利为冰岛马发布了 RI,并且在冰岛进行的一项初步研究比较了骑马马的血液学值与其他品种以及国外冰岛马的公布 RI。由于品种、性别、年龄、生殖状态和训练以及喂养、先前的运动和管理方法等因素,血液学参数可能会有很大差异。冰岛繁殖母马全年在牧场受到监管,冬季提供干草青贮料,因此,确定冰岛妊娠母马的血液学参考区间很有意义。本研究的目的是建立在牧场饲养的妊娠初期冰岛繁殖母马的血液学 RI。使用 IDEXX ProCyte Dx 分析来自 183 匹母马的稳定在 EDTA 中的血液样本,并且使用 IDEXX Catalyst One 分析仪分析了 183 匹母马中的 157 匹母马的血清样本中的总蛋白。RI 的建立是根据美国兽医临床病理学会的指南进行的。
与其他妊娠母马相比,在牧场饲养的处于妊娠早期、大多数正在哺乳的冰岛母马的红细胞计数、血细胞比容和血红蛋白 RI 较高。白细胞计数以及单核细胞、嗜酸性粒细胞和嗜碱性粒细胞的数量也是如此,在某些情况下,这可能表明一些白细胞的自动分类存在问题。
由于尚未为其他牧场饲养的冰岛马发布 RI,未来的研究应包括其他牧场饲养的冰岛马群体。这样的分析可能阐明品种、管理和妊娠对牧场饲养的冰岛马血液学值的影响。