Andriichuk A, Tkachenko H
Department of Horse Breeding, Institute of Animal Science, National Academy of Agrarian Sciences of Ukraine, Kharkiv, Ukraine.
Institute of Biology and Environmental Protection, Pomeranian University, Slupsk, Poland.
J Anim Physiol Anim Nutr (Berl). 2017 Oct;101(5):e404-e413. doi: 10.1111/jpn.12620. Epub 2017 Jan 8.
The objective of this study was to assess the influence of exercise of average intensity in the haematological and biochemical values, as well as acidic resistance of erythrocytes in mares and stallions of Holsteiner breed. A total of seventeen horses of Holstein breed (seven mares and 10 stallions aged 6 years) were used in this study. The blood samples were assessed for haematocrit (HCT) value, haemoglobin concentration (HGB), the amount of red blood cells (RBC), white blood cells (WBC), platelets (PLT), leucogram, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW) and platelet distribution width (PDW). Serum concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) as well as oxidative stress biomarkers were analysed. Stallions showed a significant increase in leucocytes and granulocytes amount, as well as erythrocytes, haemoglobin and haematocrit levels after exercise test. Pre-exercise level of mean corpuscular haemoglobin concentration was higher in stallions. At the same time, mares showed significant decrease in platelet volume after exercise test. Physical effort in stallions leads to significant increase in aspartate aminotransferase activity. Trained mares and stallions showed a decrease in lipid peroxidation after exercise. Exercise also caused increase in oxidative modified protein of erythrocytes in stallions indicating by exercise-induced oxidative stress. The resistance of erythrocytes in 0.1 m HCl was similar between females and males. No statistically significant differences in the percentage of haemolysed erythrocytes before and after exercise were observed.
本研究的目的是评估中等强度运动对荷尔斯泰因品种母马和种马血液学和生化指标以及红细胞耐酸性的影响。本研究共使用了17匹荷尔斯泰因品种的马（7匹母马和10匹6岁种马）。对血样进行了血细胞比容（HCT）值、血红蛋白浓度（HGB）、红细胞数量（RBC）、白细胞数量（WBC）、血小板数量（PLT）、白细胞分类计数、平均红细胞血红蛋白浓度（MCHC）、平均红细胞体积（MCV）、平均红细胞血红蛋白含量（MCH）、红细胞分布宽度（RDW）和血小板分布宽度（PDW）的评估。分析了血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH）的浓度以及氧化应激生物标志物。种马在运动试验后白细胞和粒细胞数量以及红细胞、血红蛋白和血细胞比容水平显著增加。种马运动前平均红细胞血红蛋白浓度较高。同时，母马在运动试验后血小板体积显著下降。种马的体力活动导致天冬氨酸转氨酶活性显著增加。经过训练的母马和种马在运动后脂质过氧化减少。运动还导致种马红细胞氧化修饰蛋白增加，表明存在运动诱导的氧化应激。雌性和雄性红细胞在0.1 m HCl中的抗性相似。运动前后溶血红细胞百分比未观察到统计学上的显著差异。
