Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, China.
Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, China.
STAR Protoc. 2024 Mar 15;5(1):102783. doi: 10.1016/j.xpro.2023.102783. Epub 2023 Dec 15.
Tumor-derived small extracellular vesicles (TEVs) play a pivotal role in cancer progression by transferring functional biomolecules between the parental and recipient cells. Here, we present a protocol to isolate TEVs directly from murine primary mammary tumor using differential centrifugation. We describe steps for tissue dissociation, enzymatic digestion, and centrifugation. We then detail procedures for characterization of TEVs through transmission electron microscopy, immunoblotting, and nano-flow cytometry. This protocol can be used to extract EVs from other solid tumor types. For complete details on the use and execution of this protocol, please refer to Li, Mei-Xin et al. (2023)..
肿瘤来源的小细胞外囊泡（TEVs）通过在亲代细胞和受体细胞之间传递功能生物分子，在癌症进展中发挥关键作用。在这里，我们提供了一种使用差速离心法直接从鼠原发性乳腺肿瘤中分离 TEV 的方案。我们描述了组织解离、酶消化和离心的步骤。然后，我们详细介绍了通过透射电子显微镜、免疫印迹和纳米流式细胞术对 TEV 进行表征的程序。该方案可用于从其他实体瘤类型中提取 EV。有关使用和执行此方案的完整详细信息，请参阅 Li, Mei-Xin 等人。（2023）。
