Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
STAR Protoc. 2024 Jun 21;5(2):102943. doi: 10.1016/j.xpro.2024.102943. Epub 2024 Mar 11.
Extracellular vesicles (EVs) play a crucial role in facilitating communication between cancer cells and their immediate or remote microenvironments, thereby promoting the extensive spread of cancer throughout the body. In this context, we present a protocol for the isolation of tumor cell-derived EVs followed by in vivo metastasis assessment in a murine ovarian cancer model. We describe steps for the isolation and characterization of EVs from ID8 cells, development of a metastatic mouse model, and sample preparation for flow cytometry. For complete details on the use and execution of this protocol, please refer to Gupta et al..
细胞外囊泡 (EVs) 在促进癌细胞与其直接或远程微环境之间的通讯方面发挥着关键作用，从而促进了癌症在全身的广泛扩散。在这种情况下，我们提出了一种从肿瘤细胞中分离 EVs 的方案，然后在小鼠卵巢癌模型中进行体内转移评估。我们描述了从 ID8 细胞中分离和表征 EVs、开发转移性小鼠模型以及为流式细胞术准备样品的步骤。有关使用和执行此方案的完整详细信息，请参阅 Gupta 等人的研究。
