使用微重力模拟装置来探究模拟微重力对K12生物膜发育的影响。
Use of a microgravity analog to explore the effects of simulated microgravity on the development of K12 biofilms.
作者信息
Hicks Janelle, Topolski Collin, Chavez Alba A, Castillo Hugo A
机构信息
Department of Human Factors and Behavioral Neurobiology, Embry-Riddle Aeronautical University, Daytona Beach, Florida, USA.
Department of Mechanical Engineering, Embry-Riddle Aeronautical University, Daytona Beach, Florida, USA.
出版信息
J Microbiol Biol Educ. 2023 Oct 27;24(3). doi: 10.1128/jmbe.00062-23. eCollection 2023 Dec.
The rapid development of space technologies and the increase of human presence in space has brought the discussion of the effects of microgravity on cells into the undergraduate classroom. This paper proposes an idea to simulate microgravity on a bacterial culture, suitable for an introductory microbiology laboratory. For this purpose, we show the use of a 2D clinostat designed for microbial studies, along with traditional microbiology techniques such as optical density, plate counts, and biofilm biomass measurement to test the effect of simulated microgravity on the growth of K12. This exercise aims to facilitate further discussions on the effects of microgravity on bacteria growth and communication, as well as the use of technology to simulate space and predict physiological changes in cells.
空间技术的迅速发展以及人类在太空中活动的增加，使得关于微重力对细胞影响的讨论进入了本科课堂。本文提出了一种在细菌培养中模拟微重力的方法，适用于微生物学入门实验室。为此，我们展示了一种专为微生物研究设计的二维回转器的使用，以及诸如光密度、平板计数和生物膜生物量测量等传统微生物学技术，以测试模拟微重力对K12生长的影响。这个实验旨在促进关于微重力对细菌生长和通讯影响的进一步讨论，以及利用技术模拟太空并预测细胞生理变化。
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