Chavez-Dozal Alba A, Nourabadi Neda, Erken Martina, McDougald Diane, Nishiguchi Michele K
Section of Infectious Diseases, New Mexico Veterans Healthcare System, Albuquerque, NM, 87108, USA.
Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, NM, 87131, USA.
Folia Microbiol (Praha). 2016 Nov;61(6):449-453. doi: 10.1007/s12223-016-0456-9. Epub 2016 Mar 24.
Multiple symbiotic and free-living Vibrio spp. grow as a form of microbial community known as a biofilm. In the laboratory, methods to quantify Vibrio biofilm mass include crystal violet staining, direct colony-forming unit (CFU) counting, dry biofilm cell mass measurement, and observation of development of wrinkled colonies. Another approach for bacterial biofilms also involves the use of tetrazolium (XTT) assays (used widely in studies of fungi) that are an appropriate measure of metabolic activity and vitality of cells within the biofilm matrix. This study systematically tested five techniques, among which the XTT assay and wrinkled colony measurement provided the most reproducible, accurate, and efficient methods for the quantitative estimation of Vibrionaceae biofilms.
多种共生和自由生活的弧菌属细菌以生物膜这种微生物群落形式生长。在实验室中，量化弧菌生物膜量的方法包括结晶紫染色、直接菌落形成单位（CFU）计数、干生物膜细胞量测量以及观察皱缩菌落的形成。另一种用于细菌生物膜的方法还涉及使用四氮唑（XTT）分析（广泛用于真菌研究），这是衡量生物膜基质中细胞代谢活性和活力的合适方法。本研究系统地测试了五种技术，其中XTT分析和皱缩菌落测量为弧菌科生物膜的定量评估提供了最可重复、准确和高效的方法。
