Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu 225009, China.
J Agric Food Chem. 2023 Dec 27;71(51):20643-20653. doi: 10.1021/acs.jafc.3c06288. Epub 2023 Dec 18.
exhibited natural resistance to a majority of succinate dehydrogenase inhibitor fungicides (SDHIs) and the molecular mechanisms responsible for the natural resistance were still unknown. Succinate dehydrogenase subunit C () is an essential gene for maintaining succinate-ubiquinone oxidoreductase (SQR) function in fungi. In , a paralog of named as was identified. Based on RNA-Seq and qRT-PCR assay, we found that the expression level of was very low but upregulated by SDHIs treatment. Based on reverse genetics, we demonstrated that was an inessential gene in normal growth but was sufficient for maintaining SQR function and conferred natural resistance or reduced sensitivity toward SDHIs. Additionally, we found that the standard isolate PH-1 had high sensitivity to a majority of SDHIs. A single nucleotide variation (C to T) in the of isolate PH-1, resulting in a premature termination codon (TAA) replacing the fourth amino acid glutamine (Q), led to the failure of to perform functions of conferring nature resistance. These results established that a dispensable paralogous gene determined SDHIs resistance in natural populations of .
表现出对大多数琥珀酸脱氢酶抑制剂（SDHI）杀菌剂的天然抗性，但其天然抗性的分子机制尚不清楚。琥珀酸脱氢酶亚基 C（）是真菌中维持琥珀酸-泛醌氧化还原酶（SQR）功能的必需基因。在中，鉴定出一个名为的的基因。基于 RNA-Seq 和 qRT-PCR 分析，我们发现的表达水平非常低，但被 SDHIs 处理上调。基于反向遗传学，我们证明在正常生长中是一个非必需基因，但足以维持 SQR 功能，并赋予对 SDHIs 的天然抗性或降低敏感性。此外，我们发现标准 分离株 PH-1 对大多数 SDHIs 高度敏感。分离株 PH-1 中 的一个单核苷酸变异（C 到 T），导致提前终止密码子（TAA）取代第四个氨基酸谷氨酰胺（Q），导致无法发挥赋予天然抗性的功能。这些结果确立了一个可有可无的基因决定了自然种群中对 SDHIs 的抗性。
