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FpFumB通过与FpSae2相互作用调节DNA修复，对其基本生物学过程和毒力是必需的。 （注：原文中“in by”表述有误，推测可能是“in”，翻译按照修正后的内容进行）

FpFumB Is Required for Basic Biological Processes and Virulence in by Modulating DNA Repair Through Interaction with FpSae2.

作者信息

Gao Yizhou, Li Haibo, Liu Yong, Wang Yuqing, Xue Jingwen, Wang Yitong, Wu Zhihong

机构信息

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.

出版信息

Microorganisms. 2025 Jun 19;13(6):1433. doi: 10.3390/microorganisms13061433.

DOI:10.3390/microorganisms13061433
PMID:40572321
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12196060/
Abstract

Fumarase plays a pivotal role in the tricarboxylic acid cycle, but its functions in plant pathogenic fungi are not well understood. We identified two fumarase genes in and generated individual deletion mutants. Loss of led to defects in growth, sporulation, stress tolerance, and virulence. Exogenous malate supplementation restored growth defects. Site-directed mutagenesis of residues G452 and A463 reduced FpFumB enzyme activity. Transcriptomic analysis identified significant changes in gene expression related to different metabolic pathways. Protein interaction assays showed that FpFumB interacts with the DNA repair protein FpSae2. Both Δ and Δ mutants displayed altered sensitivity to DNA-damaging agents and reduced virulence, indicating that FpFumB modulates DNA repair and pathogenicity through its interaction with FpSae2. Together, these findings highlight FpFumB as a key regulator of basic biological processes, DNA damage repair, and virulence in .

摘要

延胡索酸酶在三羧酸循环中起关键作用，但其在植物病原真菌中的功能尚未完全明确。我们在[具体物种]中鉴定出两个延胡索酸酶基因，并构建了单个基因缺失突变体。[基因缺失]导致生长、孢子形成、胁迫耐受性和毒力方面出现缺陷。外源添加苹果酸可恢复生长缺陷。对G452和A463位点进行定点诱变降低了FpFumB酶活性。转录组分析确定了与不同代谢途径相关的基因表达存在显著变化。蛋白质相互作用分析表明FpFumB与DNA修复蛋白FpSae2相互作用。Δ[具体突变体1]和Δ[具体突变体2]突变体对DNA损伤剂的敏感性均发生改变且毒力降低，表明FpFumB通过与FpSae2相互作用调节DNA修复和致病性。总之，这些发现突出了FpFumB作为[具体物种]中基本生物学过程、DNA损伤修复和毒力的关键调节因子的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/1f66a6086cdb/microorganisms-13-01433-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/e4e4055bd2fc/microorganisms-13-01433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/e8e6d03de7f9/microorganisms-13-01433-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/d352eed04de2/microorganisms-13-01433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/75a49ac18d06/microorganisms-13-01433-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/a08f51dd5d72/microorganisms-13-01433-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/1e9d2a509908/microorganisms-13-01433-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/181e41b4b424/microorganisms-13-01433-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/1f66a6086cdb/microorganisms-13-01433-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/e4e4055bd2fc/microorganisms-13-01433-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/e8e6d03de7f9/microorganisms-13-01433-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/d352eed04de2/microorganisms-13-01433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/75a49ac18d06/microorganisms-13-01433-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/a08f51dd5d72/microorganisms-13-01433-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/1e9d2a509908/microorganisms-13-01433-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/181e41b4b424/microorganisms-13-01433-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b2/12196060/1f66a6086cdb/microorganisms-13-01433-g008.jpg

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本文引用的文献

[1]
Paralog Confers Natural Resistance toward SDHI Fungicides in .

J Agric Food Chem. 2023-12-27

[2]
The Pleiotropic Effects of Fumarate: From Mitochondrial Respiration to Epigenetic Rewiring and DNA Repair Mechanisms.

Metabolites. 2023-7-24

[3]
Cross-talk between DNA damage response and the central carbon metabolic network underlies selective vulnerability of Purkinje neurons in ataxia-telangiectasia.

J Neurochem. 2023-8

[4]
FonTup1 functions in growth, conidiogenesis and pathogenicity of Fusarium oxysporum f. sp. niveum through modulating the expression of the tricarboxylic acid cycle genes.

Microbiol Res. 2023-7

[5]
Fumonisin B1 Biosynthesis Is Associated with Oxidative Stress and Plays an Important Role in Infection on Banana Fruit.

J Agric Food Chem. 2023-4-5

[6]
Fusarium oxysporum f. sp. Pumilio 1 Regulates Virulence on Watermelon through Interacting with the ARP2/3 Complex and Binding to an A-Rich Motif in the 3' UTR of Diverse Transcripts.

mBio. 2023-4-25

[7]
Strategies for Controlling the Sporulation in spp.

J Fungi (Basel). 2022-12-21

[8]
The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6.

J Integr Plant Biol. 2023-3

[9]
Cytosolic fumarase acts as a metabolic fail-safe for both high and low temperature acclimation of Arabidopsis thaliana.

J Exp Bot. 2022-4-5

[10]
Fumarase affects the deoxyribonucleic acid damage response by protecting the mitochondrial desulfurase Nfs1p from modification and inactivation.

iScience. 2021-10-26

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