Department of Respiratory Medicine, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.
ASKA Biotech GmbH Veltener Str. 12, Hennigsdorf, Germany.
Methods Mol Biol. 2024;2750:113-122. doi: 10.1007/978-1-0716-3605-3_11.
Enzyme-linked immunosorbent assay (ELISA) is a sensitive immunoassay based on specific antigen-antibody reaction that is used for quantitative/qualitative analysis of various analytes in serum, plasma, saliva, cell and tissue lysates, and urine. ELISAs are typically performed in multi-well plates and depending on the design require coating antibody/antigen, analyte, detection antibodies, buffer, wash solution, and substrate/chromogen. Here we describe highly specific monoclonal antibody-based ELISA that detects circulating polymers formed by PiZ variant of human alpha-1-antitrypsin (Z-AAT). The circulating Z-AAT polymers are present in all individuals with inherited PiZ AAT deficiency. Thus, our assay provides a useful tool to examine the clinical significance and utility of Z-AAT polymers.
酶联免疫吸附测定（ELISA）是一种基于特异性抗原-抗体反应的灵敏免疫测定法，用于定量/定性分析血清、血浆、唾液、细胞和组织裂解物以及尿液中的各种分析物。ELISA 通常在微孔板中进行，具体取决于设计要求，包括包被抗体/抗原、分析物、检测抗体、缓冲液、洗涤溶液和底物/显色剂。在此，我们描述了一种基于高度特异性单克隆抗体的 ELISA 检测方法，该方法可检测人α-1-抗胰蛋白酶 PiZ 变异体（Z-AAT）形成的循环聚合物。所有遗传性 PiZ AAT 缺乏症个体中均存在循环 Z-AAT 聚合物。因此，我们的测定法为研究 Z-AAT 聚合物的临床意义和用途提供了有用的工具。
