COLEC12 促进胃癌进展并与不良预后相关。
COLEC12 Promotes Tumor Progression and Is Correlated With Poor Prognosis in Gastric Cancer.
机构信息
Department of General Surgery, Zhongshan Hospital, Fudan University School of Medicine, Shanghai, China.
出版信息
Technol Cancer Res Treat. 2023 Jan-Dec;22:15330338231218163. doi: 10.1177/15330338231218163.
PURPOSE
Collectin subfamily member 12, a transmembrane scavenger receptor C-type lectin, is aberrantly expressed in various cancers. However, its physiological role in gastric cancer remains somewhat unclear. This study aimed to investigate the Collectin subfamily member 12 expression pattern in human gastric cancer and its role in gastric cancer progression.
METHODS
The Kaplan-Meier method was used for survival analysis. The univariate and multivariate Cox proportional hazards regression models were used to identify independent predictors for progression-free survival and overall survival. The effects of Collectin subfamily member 12 on gastric cancer cell proliferation, migration, invasion, and apoptosis were detected through the cell counting kit-8 assay, colony formation assay, wound healing assay, transwell assay, and flow cytometry analysis, respectively. Additionally, the correlation between Collectin subfamily member 12 expression and immune cell infiltration was analyzed through bioinformatics.
RESULTS
Collectin subfamily member 12 was highly expressed in advanced gastric cancer (T3-T4, pathologic stage III-IV). High Collectin subfamily member 12 expression was correlated with a worse progression-free survival and overall survival in the gastric cancer patients. , cell line studies revealed that Collectin subfamily member 12 promoted gastric cancer cell proliferation, migration, and invasion and inhibited gastric cancer cell apoptosis. The bioinformatics analysis further demonstrated that the Collectin subfamily member 12 expression level positively correlated with infiltration of several immune cells, such as M2 macrophages, dendritic cells, neutrophils, and regulatory T cells, suggesting that Collectin subfamily member 12 may also play a role in suppressing tumor immune response in gastric cancer.
CONCLUSIONS
Collectin subfamily member 12 was identified as a novel predictive marker and target for the clinical treatment of gastric cancer.
目的
胶原凝集素亚家族成员 12 是一种跨膜清道夫受体 C 型凝集素，在多种癌症中异常表达。然而，其在胃癌中的生理作用尚不清楚。本研究旨在探讨胶原凝集素亚家族成员 12 在人胃癌中的表达模式及其在胃癌进展中的作用。
方法
采用 Kaplan-Meier 法进行生存分析。采用单因素和多因素 Cox 比例风险回归模型确定无进展生存期和总生存期的独立预测因子。通过细胞计数试剂盒-8 检测、集落形成实验、划痕愈合实验、Transwell 检测和流式细胞术分析分别检测胶原凝集素亚家族成员 12 对胃癌细胞增殖、迁移、侵袭和凋亡的影响。此外，通过生物信息学分析，分析胶原凝集素亚家族成员 12 表达与免疫细胞浸润的相关性。
结果
胶原凝集素亚家族成员 12 在晚期胃癌（T3-T4，病理分期 III-IV）中高表达。高表达胶原凝集素亚家族成员 12 与胃癌患者无进展生存期和总生存期较差相关。细胞系研究表明，胶原凝集素亚家族成员 12 促进胃癌细胞增殖、迁移和侵袭，抑制胃癌细胞凋亡。生物信息学分析进一步表明，胶原凝集素亚家族成员 12 的表达水平与 M2 巨噬细胞、树突状细胞、中性粒细胞和调节性 T 细胞等几种免疫细胞的浸润呈正相关，提示胶原凝集素亚家族成员 12 可能在抑制胃癌肿瘤免疫反应中发挥作用。
结论
胶原凝集素亚家族成员 12 被鉴定为胃癌临床治疗的新预测标志物和靶标。
Zotero 插件