Oehmichen M, Grüninger H, Norpoth T
Z Rechtsmed. 1986;97(2):117-31. doi: 10.1007/BF00204445.
The time-dependent acellular, destructive changes in erythrocytes was investigated in rabbits by subcutaneous, intraperitoneal, and subdural implantation of blood-filled diffusion chambers with 0.45 micron pores. The animals were killed at various intervals after implantation (7-24 h), and the erythrocytes in the capsules were examined by light and electron microscopy. The findings were quantified by counting 100 cells per preparation under the light microscope; changes in both matrix and membrane structure were recorded. Matrix changes and membrane variants were specified by electron microscopy. Time-dependent changes in erythrocyte matrix and membrane were established, which occurred at the various localizations in an identical sequence, but with different time intervals. The destructive changes were: an increase in loss of matrix, increase in membrane permeability, and decrease in membrane stability. The physiologic bases of and the conclusions for wound timing were discussed.
通过皮下、腹腔内和硬膜下植入带有0.45微米孔径的充满血液的扩散室，研究了家兔红细胞随时间变化的脱细胞、破坏性变化。在植入后不同时间间隔（7 - 24小时）处死动物，并通过光学显微镜和电子显微镜检查胶囊内的红细胞。通过在光学显微镜下每个标本计数100个细胞对结果进行量化；记录基质和膜结构的变化。通过电子显微镜确定基质变化和膜变异。确定了红细胞基质和膜随时间的变化，这些变化在不同部位以相同顺序发生，但时间间隔不同。破坏性变化包括：基质损失增加、膜通透性增加和膜稳定性降低。讨论了伤口时机的生理基础和结论。
