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使用抗人血清通过酶联免疫吸附测定(ELISA)对血迹中的人类和黑猩猩进行区分。

Differentiation between human and chimpanzee in bloodstains by enzyme-linked immunosorbent assay (ELISA) using antihuman serum.

作者信息

Tsutsumi H, Sato M, Nakamura S, Katsumata Y

出版信息

Z Rechtsmed. 1986;97(2):99-103. doi: 10.1007/BF00204442.

DOI:10.1007/BF00204442
PMID:3811632
Abstract

An enzyme-linked immunosorbent assay (ELISA) for species identification of human bloodstains using two commercially available antisera against human serum is described. Human bloodstains were to be distinguished from those of chimpanzees and other animals using raw antisera, and the differentiation between human and chimpanzee became more evident when those antisera were absorbed with a small amount of chimpanzee plasma. Human bloodstains could clearly be identified by the present method even after 4 weeks of aging at room temperature.

摘要

描述了一种使用两种市售抗人血清抗血清进行人血迹物种鉴定的酶联免疫吸附测定(ELISA)。使用未处理的抗血清将人血迹与黑猩猩和其他动物的血迹区分开来,当这些抗血清用少量黑猩猩血浆吸收后,人与黑猩猩之间的差异变得更加明显。即使在室温下老化4周后,通过本方法也能清楚地鉴定出人血迹。

相似文献

1
Differentiation between human and chimpanzee in bloodstains by enzyme-linked immunosorbent assay (ELISA) using antihuman serum.使用抗人血清通过酶联免疫吸附测定(ELISA)对血迹中的人类和黑猩猩进行区分。
Z Rechtsmed. 1986;97(2):99-103. doi: 10.1007/BF00204442.
2
Identification of human bloodstains by enzyme-linked immunosorbent assay (ELISA).
Nihon Hoigaku Zasshi. 1983 Apr;37(2):84-7.
3
Antigenic properties of human and animal bloodstains studied by enzyme-linked immunosorbent assay (ELISA) using various antisera against specific plasma proteins.采用针对特定血浆蛋白的各种抗血清,通过酶联免疫吸附测定(ELISA)研究人和动物血迹的抗原特性。
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[Detection of G2m(n) factor in bloodstains using ELISA inhibition test].[采用酶联免疫吸附抑制试验检测血迹中的G2m(n)因子]
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引用本文的文献

1
Antigenic properties of human and animal bloodstains studied by enzyme-linked immunosorbent assay (ELISA) using various antisera against specific plasma proteins.采用针对特定血浆蛋白的各种抗血清,通过酶联免疫吸附测定(ELISA)研究人和动物血迹的抗原特性。
Z Rechtsmed. 1987;99(3):191-6. doi: 10.1007/BF00201250.
2
Rapid and sensitive identification of human blood by an ELISA-ABC method using a biotinylated antibody against human HbA0.使用抗人血红蛋白A0生物素化抗体的ELISA-ABC法对人血进行快速灵敏鉴定。
Z Rechtsmed. 1990;103(5):329-34. doi: 10.1007/BF01263037.
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Application of ELISA-ABC method to the identification of minute human bloodstains.

本文引用的文献

1
The antigenicity of sera of man and animals in relation to the preparation of specific precipitating antisera.人与动物血清的抗原性与特异性沉淀抗血清制备的关系。
J Hyg (Lond). 1952 Sep;50(3):275-94. doi: 10.1017/s0022172400019616.
2
Species identification of blood and saliva stains by enzyme-linked immunoassay (ELISA) using monoclonal antibody.
J Forensic Sci. 1984 Jan;29(1):67-74.
3
[Identification of human hemoglobin by enzyme-linked immunosorbent assay (ELISA) using hemoglobin-coated polystyrene beads (II). Forensic immunological studies on human specificity, report-XVII].
Nihon Hoigaku Zasshi. 1984 Aug;38(4):403-9.
4
ELISA-ABC法在微量人血痕鉴定中的应用。
Int J Legal Med. 1991;104(3):123-6. doi: 10.1007/BF01369714.
[Identification of human hemoglobin by enzyme-linked immunosorbent assay (ELISA) using hemoglobin-coated polystyrene beads--forensic immunological studies on human specificity. Report-XVI].
Nihon Hoigaku Zasshi. 1984 Apr;38(2):170-6.
5
Identification of human bloodstains by enzyme-linked immunosorbent assay (ELISA).
Nihon Hoigaku Zasshi. 1983 Apr;37(2):84-7.