Ophthalmology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, United States.
State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Zhejiang, China.
Invest Ophthalmol Vis Sci. 2023 Dec 1;64(15):26. doi: 10.1167/iovs.64.15.26.
To investigate the flow pattern in unconventional outflow and its correlation with conventional outflow in mouse eyes.
Fluorescent microspheres were injected into the anterior chamber of one eye of anesthetized C57BL/6J mice (n = 4), followed by perfused fixation with 4% paraformaldehyde in situ after 45 minutes. Post-euthanasia, the injected eyes were enucleated, further immersion fixed, and dissected into 12 equal radial segments. Both sides of each segment were imaged using a confocal microscope after nuclear counterstaining. Both unconventional and conventional outflow patterns of each eye were analyzed by ImageJ and ZEN 2.3 imaging software.
Segmental outflow patterns were observed in both the ciliary body (CB) and the supraciliary space and suprachoroidal space (SCS). In the CB, the tracer intensity was the lowest at 12 o'clock and highest at 9 o'clock, whereas in the SCS it was the lowest at 2 o'clock and the highest at 10 o'clock. Consequently, a segmental unconventional outflow was observed, with the lowest and highest flow regions in the superior and temporal quadrants, respectively. The overall segmental uveoscleral outflow has no correlation with trabecular outflow (P > 0.05). Four different outflow patterns were observed: (1) low-flow regions in both outflows, (2) primarily a high-flow region in conventional outflow, (3) primarily a high-flow region in unconventional outflow, and (4) high-flow regions in both outflows.
Uveoscleral outflow is segmental and unrelated to the trabecular segmental outflow. These findings will lead to future studies to identify the best location for the placement of drainage devices and drug delivery.
研究非传统流出道中的流动模式及其与小鼠眼睛中传统流出道的相关性。
将荧光微球注入麻醉的 C57BL/6J 小鼠一只眼的前房（n = 4），45 分钟后用 4%多聚甲醛原位灌流固定。安乐死后，将注射眼眼球摘除，进一步浸泡固定，沿 12 个相等的放射状段进行解剖。用共聚焦显微镜对每个节段的两侧进行核染色后成像。用 ImageJ 和 ZEN 2.3 成像软件分析每只眼的非传统和传统流出模式。
在睫状体（CB）和睫上间隙以及脉络膜上腔（SCS）中均观察到节段性流出模式。在 CB 中，示踪剂强度在 12 点最低，9 点最高，而在 SCS 中，2 点最低，10 点最高。因此，观察到节段性非传统流出，其最高和最低流动区域分别在上方和颞象限。整体节段性葡萄膜巩膜流出与小梁流出无相关性（P > 0.05）。观察到四种不同的流出模式：（1）两种流出均为低流区，（2）主要为常规流出的高流区，（3）主要为非传统流出的高流区，（4）两种流出均为高流区。
葡萄膜巩膜流出是节段性的，与小梁节段性流出无关。这些发现将导致未来的研究，以确定放置引流装置和药物输送的最佳位置。
