Beltramini-Sabbag L M, Krieger E M, Greene L J, Reis M L
Adv Exp Med Biol. 1986;198 Pt B:289-96. doi: 10.1007/978-1-4757-0154-8_36.
We have extended our previous study of increased levels of new spasmogenic substances released by trypsin from the plasma of chronic one-kidney, one-clip hypertensive rats by describing preparative procedures utilizing Sephadex G-25, CM-cellulose and C18 reverse-phase HPLC for their partial purification. We demonstrate the existence of at least 6 HPLC components, distinguishable from bradykinin, bradykinin homologues having Lys before Arg1 of bradykinin and T-kinin (Ile-Ser-bradykinin) on the basis of their chromatographic properties. Several of these spasmogenic substances induce a very slow contraction of the isolated guinea pig ileum when compared to bradykinin.
我们扩展了之前关于慢性单肾单夹高血压大鼠血浆中胰蛋白酶释放的新致痉物质水平升高的研究，描述了利用葡聚糖凝胶G - 25、CM - 纤维素和C18反相高效液相色谱进行部分纯化的制备程序。我们证明至少存在6种高效液相色谱成分，根据其色谱特性，它们与缓激肽、缓激肽前体（缓激肽的精氨酸1之前有赖氨酸）和T - 激肽（异亮氨酸 - 丝氨酸 - 缓激肽）不同。与缓激肽相比，其中几种致痉物质可诱导离体豚鼠回肠非常缓慢的收缩。
