Gottschall J L, Collins J, Kunicki T J, Nash R, Aster R H
Am J Clin Pathol. 1987 Feb;87(2):218-22. doi: 10.1093/ajcp/87.2.218.
Addition of red blood cells hemolyzed in the presence of dilute plasma in quantities equivalent to as little as 0.2% hemolysis of red blood cells in whole blood caused significant elevation in the apparent value of IgG associated with platelets (PAIgG) isolated from the preparation. Addition of hemolysate to plasma containing low concentrations of platelets, simulating that obtained from thrombocytopenic patients, caused more striking elevations in apparent PAIgG. Centrifugation of platelet-rich plasma containing hemolysate through a 30% solution of Percoll restored the value of PAIgG essentially to normal. Additional studies using simulated patient whole blood samples anticoagulated with EDTA yielded similar results. The apparent elevation of PAIgG in these preparations appears to result from IgG-bearing membranous material and microspherocytes that co-isolate with platelets separated from blood by conventional methods. Caution should be used in interpreting the results of PAIgG measurements on platelets isolated from blood samples in which even minimal in vitro hemolysis has occurred.
在存在稀释血浆的情况下，添加相当于全血中红细胞溶血低至0.2%量的溶血红细胞，会导致从制备物中分离出的与血小板相关的IgG（PAIgG）表观值显著升高。将溶血产物添加到含有低浓度血小板的血浆中（模拟血小板减少症患者获得的血浆），会使表观PAIgG升高更为显著。通过30%的Percoll溶液对含有溶血产物的富含血小板血浆进行离心，可使PAIgG值基本恢复正常。使用用EDTA抗凝的模拟患者全血样本进行的其他研究也得出了类似结果。这些制备物中PAIgG的表观升高似乎是由于与通过传统方法从血液中分离出的血小板共同分离的携带IgG的膜性物质和微球红细胞所致。在解释从即使发生了最小程度体外溶血的血样中分离出的血小板的PAIgG测量结果时应谨慎。
