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Micro-enzyme-linked immunoassay for the quantitation of platelet-associated IgG (PAIgG).

作者信息

Forster J, Schmidt B

出版信息

Klin Wochenschr. 1983 Feb 1;61(3):165-7. doi: 10.1007/BF01486372.

DOI:10.1007/BF01486372
PMID:6341701
Abstract

An enzyme linked immunoassay was developed using the microtiter system to measure platelet-associated IgG (PAIgG). One single determination needs 2 x 10(6) platelets usually obtained from 3 ml blood anticoagulated with EDTA. Platelets are incubated with peroxidase-conjugated anti-human IgG. Its unbound fraction is adsorbed to an IgG coated microtiter plate, quantitated by a colour reaction and found to be inversely related to the amount of PAIgG. Healthy donors (n = 40, aged 1 day to 35 years) possessed 3.6 +/- 2.0 (mean +/- S.D.) fg IgG/platelet. Increased levels of PAIgG were found in patients with acute (n = 16) and chronic (n = 5) idiopathic thrombocytopenic purpura. Postrecovery platelets had normal values of PAIgG. Widely varying levels of PAIgG were found in patients with freshly diagnosed acute lymphocytic leukemia.

摘要

相似文献

1
Micro-enzyme-linked immunoassay for the quantitation of platelet-associated IgG (PAIgG).
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2
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Am J Clin Pathol. 1984 Jan;81(1):81-4. doi: 10.1093/ajcp/81.1.81.
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A quantitative ELISA procedure for the measurement of membrane-bound platelet-associated IgG (PAIgG).一种用于测定膜结合血小板相关免疫球蛋白G（PAIgG）的定量酶联免疫吸附测定方法。
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Acta Haematol. 1981;66(4):251-6. doi: 10.1159/000207130.
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Direct quantitation of platelet-associated IgG by electroimmunoassay.用电免疫测定法直接定量血小板相关IgG。
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Platelet-associated immunoglobulin G in childhood idiopathic thrombocytopenic purpura.儿童特发性血小板减少性紫癜中的血小板相关免疫球蛋白G
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引用本文的文献

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Successful therapy of sarcoidosis-associated thrombocytopenia refractory to corticosteroids by a single course of human gammaglobulins.单疗程人丙种球蛋白成功治疗对皮质类固醇难治的结节病相关性血小板减少症。
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3
Relative importance of total versus external platelet-associated IgG.总血小板相关IgG与外源性血小板相关IgG的相对重要性。

本文引用的文献

1
Relationship of raised platelet IgG in thrombocytopenia to total platelet protein content.血小板减少症中升高的血小板IgG与血小板总蛋白含量的关系。
Br J Haematol. 1981 Oct;49(2):293-302. doi: 10.1111/j.1365-2141.1981.tb07226.x.
2
Enzyme linked immunoassay for the detection of platelet associated IgG.
Br J Haematol. 1981 May;48(1):39-46. doi: 10.1111/j.1365-2141.1981.00039.x.
3
The clinical significance of platelet-associated IgG: a study on 298 patients with various disorders.血小板相关IgG的临床意义：对298例患有各种疾病患者的研究。
Blut. 1989 Jul;59(1):67-74. doi: 10.1007/BF00320252.
Br J Haematol. 1980 Sep;46(1):123-31. doi: 10.1111/j.1365-2141.1980.tb05942.x.
4
Immune complexes in thrombocytopenic patients: cause or effect?血小板减少症患者体内的免疫复合物：是病因还是结果？
Br J Haematol. 1980 Apr;44(4):645-54. doi: 10.1111/j.1365-2141.1980.tb08719.x.
5
A new method for the simultaneous quantitation of platelet-bound immunoglobuin (IgG) and complement (C3) employing an enzyme-linked immunosorbent assay (ELISA) procedure.
Br J Haematol. 1980 Feb;44(2):281-90. doi: 10.1111/j.1365-2141.1980.tb01210.x.
6
Simultaneous enzymo-immunologic assays of platelet associated IgG, IgM and C3. A useful tool in assessment of immune thrombocytopenias.血小板相关IgG、IgM和C3的同步酶免疫测定。评估免疫性血小板减少症的有用工具。
Thromb Res. 1982 May 15;26(4):249-58. doi: 10.1016/0049-3848(82)90289-4.
7
Quantification of platelet-associated IgG with competitive solid-phase enzyme immunoassay.采用竞争性固相酶免疫测定法对血小板相关IgG进行定量分析。
Acta Haematol. 1981;66(4):251-6. doi: 10.1159/000207130.
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Quantitation of platelet-associated IgG by radial immunodiffusion.通过放射免疫扩散法定量检测血小板相关IgG
Blood. 1981 Apr;57(4):809-11.
9
Detection of anti-platelet antibodies in patients with idiopathic thrombocytopenic purpura (ITP) and in patients with rubella and herpes group viral infections.特发性血小板减少性紫癜（ITP）患者以及风疹和疱疹病毒感染患者中抗血小板抗体的检测。
Clin Exp Immunol. 1981 Apr;44(1):49-56.
10
Increased platelet-associated IgG in child on high-dose gammaglobulin for idiopathic thrombocytopenic purpura.接受大剂量丙种球蛋白治疗特发性血小板减少性紫癜的儿童血小板相关IgG升高。
Lancet. 1982 Jul 3;2(8288):39-40. doi: 10.1016/s0140-6736(82)91168-0.