Köhler E, Beglinger C, Eysselein V, Grötzinger U, Gyr K
Am J Physiol. 1987 Jan;252(1 Pt 1):G40-4. doi: 10.1152/ajpgi.1987.252.1.G40.
The role of gastrin as a regulator of exocrine pancreatic secretion has not been proven adequately. In the present study we therefore compared the relative molar potencies of sulfated and unsulfated gastrin 17 with structurally related CCK peptides (synthetic CCK-8 and natural porcine CCK-33) in stimulating exocrine pancreatic secretion in conscious dogs. Dose response curves were constructed for pancreatic and gastric acid secretion. Plasma gastrin levels after exogenous gastrin 17-I and -II were compared with postprandial gastrin concentrations (meal: ground beef 20 g/kg body wt). The molar potency estimates calculated with synthetic CCK8 as standard (potency = 1.00) for pancreatic protein secretion were natural porcine 50% pure CCK-33 1.60, gastrin 17-I 0.12, and gastrin 17-II 0.16. All four peptides induced a dose-dependent increase in pancreatic bicarbonate output. However, the blood concentrations needed to stimulate pancreatic secretion were above the postprandial gastrin levels. Our data indicate that both gastrin 17 peptides are not physiological regulators of pancreatic enzyme secretion in dogs.
胃泌素作为外分泌性胰腺分泌调节因子的作用尚未得到充分证实。因此，在本研究中，我们比较了硫酸化和未硫酸化的胃泌素17与结构相关的胆囊收缩素肽（合成CCK-8和天然猪CCK-33）在刺激清醒犬外分泌性胰腺分泌方面的相对摩尔效力。构建了胰腺和胃酸分泌的剂量反应曲线。将外源性胃泌素17-I和-II后的血浆胃泌素水平与餐后胃泌素浓度进行比较（餐食：每千克体重20克碎牛肉）。以合成CCK8为标准（效力 = 1.00）计算的胰腺蛋白质分泌的摩尔效力估计值为：天然猪50%纯CCK-33为1.60，胃泌素17-I为0.12，胃泌素17-II为0.16。所有四种肽均引起胰腺碳酸氢盐输出的剂量依赖性增加。然而，刺激胰腺分泌所需的血药浓度高于餐后胃泌素水平。我们的数据表明，两种胃泌素17肽不是犬胰腺酶分泌的生理调节因子。
