从牛心肌丙酮粉中提取肌球蛋白轻链和肌动蛋白。

Extraction of myosin light chains and actin from bovine cardiac muscle acetone powder.

作者信息

Barden J A, Symons P, Cartmill J A, Miki M, Hambly B D

出版信息

Anal Biochem. 1986 Nov 1;158(2):288-93. doi: 10.1016/0003-2697(86)90552-x.

Abstract

A joint preparation of the two myosin light chains and actin from bovine cardiac muscle acetone powder is described. There is a significant improvement in the ease of purification, while the yield of the myosin light chains equals the best yields obtained from the use of established techniques. The actin yield greatly exceeds that obtained in an earlier published report.

摘要

本文描述了一种从牛心肌丙酮粉中制备两种肌球蛋白轻链与肌动蛋白的联合制剂。在纯化的简便性方面有显著改进，而肌球蛋白轻链的产量与使用既定技术所获得的最佳产量相当。肌动蛋白的产量大大超过了早期发表报告中的产量。