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高效液相色谱法和紫外分光光度法用于测定钡水解产物中的色氨酸含量。

High-performance liquid chromatography and ultraviolet spectrophotometry for quantitation of tryptophan in barytic hydrolysates.

作者信息

Delhaye S, Landry J

出版信息

Anal Biochem. 1986 Nov 15;159(1):175-8. doi: 10.1016/0003-2697(86)90324-6.

DOI:10.1016/0003-2697(86)90324-6
PMID:3812997
Abstract

A procedure for quantitation of tryptophan in feedstuffs is described. It is based on barytic hydrolysis of material at 125 degrees C for 16 h, acidification of hydrolysate to pH 3 with HCl, high-performance liquid chromatography on Nova Pak C18 (Waters Assoc.), and spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme added to samples ranges from 98.7 to 100%.

摘要

描述了一种饲料中色氨酸的定量方法。该方法基于在125℃下对样品进行16小时的钡水解，用盐酸将水解产物酸化至pH 3，在Nova Pak C18（沃特世公司）上进行高效液相色谱分析，并在280nm处用分光光度法测定色氨酸。添加到样品中的溶菌酶中色氨酸的回收率在98.7%至100%之间。

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引用本文的文献

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HPLC determination of tryptophan in foodstuffs using barium hydroxide hydrolysis at 140°C.采用 140°C 氢氧化钡水解法的高效液相色谱法测定食品中的色氨酸。
Amino Acids. 1993 Jun;5(2):317-21. doi: 10.1007/BF00805993.
2
Manipulation of amino acid composition in soybean seeds by the combination of deregulated tryptophan biosynthesis and storage protein deficiency.通过调控色氨酸生物合成和贮存蛋白缺失来操纵大豆种子中的氨基酸组成。
Plant Cell Rep. 2010 Jan;29(1):87-95. doi: 10.1007/s00299-009-0800-5. Epub 2009 Nov 27.
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Biosynthesis and characterization of 4-fluorotryptophan-labeled Escherichia coli arginyl-tRNA synthetase.
4-氟色氨酸标记的大肠杆菌精氨酰-tRNA合成酶的生物合成与表征
J Protein Chem. 1999 Feb;18(2):187-92. doi: 10.1023/a:1020675922382.