• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Sja-Let-7 通过 Col1α2 减轻小鼠模型中四氯化碳诱导的肝纤维化。

Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1α2.

作者信息

Zhong Haoran, Dong Bowen, Zhu Danlin, Li Hao, Lu Ke, Fu Zhiqiang, Liu Jinming, Jin Yamei

机构信息

National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.

Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.

出版信息

Biology (Basel). 2023 Nov 24;12(12):1465. doi: 10.3390/biology12121465.

DOI:10.3390/biology12121465
PMID:38132291
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10740823/
Abstract

Liver fibrosis (LF) is a chronic progressive disease with no definitive treatment. The aim of this study was to assess helminth-derived molecules as potential therapeutic targets to prevent or reverse LF. A mouse model of carbon tetrachloride (CCL)-induced LF was established and sja-let-7 was overexpressed by treatment with a miRNA agomir once per week. After four weeks, serum biochemistry, hepatic hydroxyproline content measurements, liver histology, mRNA expression profiling of fibrotic markers, the dual-luciferase reporter assay, and fluorescence in situ hybridization (FISH) were performed. Administration of the sja-let-7 agomir markedly ameliorated hepatosplenomegaly and reduced the liver hydroxyproline content. Liver histological analysis showed significant reductions in collagen deposition in the sja-let-7 agomir-treated mice. Additionally, the mRNA levels of both pro-fibrotic markers and pro-inflammatory cytokines were diminished after treatment. Furthermore, the dual-luciferase reporter assay and FISH identified the α2 chain of collagen type 1 (Col1α2) as the direct target of sja-let-7. Accordingly, the progression of LF was attenuated by targeting Col1α2 and the TGF-β/Smad signaling pathway.

摘要

肝纤维化（LF）是一种尚无确切治疗方法的慢性进行性疾病。本研究的目的是评估源自蠕虫的分子作为预防或逆转肝纤维化的潜在治疗靶点。建立了四氯化碳（CCL）诱导的肝纤维化小鼠模型，并通过每周一次用miRNA激动剂处理使sja-let-7过表达。四周后，进行了血清生化分析、肝脏羟脯氨酸含量测定、肝脏组织学检查、纤维化标志物的mRNA表达谱分析、双荧光素酶报告基因检测以及荧光原位杂交（FISH）。给予sja-let-7激动剂可显著改善肝脾肿大，并降低肝脏羟脯氨酸含量。肝脏组织学分析显示，经sja-let-7激动剂处理的小鼠胶原沉积明显减少。此外，治疗后促纤维化标志物和促炎细胞因子的mRNA水平均降低。此外，双荧光素酶报告基因检测和FISH确定Ⅰ型胶原α2链（Col1α2）为sja-let-7的直接靶点。因此，通过靶向Col1α2和TGF-β/Smad信号通路可减轻肝纤维化的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/3897c6010e1f/biology-12-01465-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/fff1a8314ba8/biology-12-01465-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/3f256d460685/biology-12-01465-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/b988ca5ccf0b/biology-12-01465-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/4fcf2c6bf286/biology-12-01465-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/6d0c143be20b/biology-12-01465-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/1fd470800255/biology-12-01465-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/273b6d034563/biology-12-01465-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/c7002d96a2f4/biology-12-01465-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/3897c6010e1f/biology-12-01465-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/fff1a8314ba8/biology-12-01465-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/3f256d460685/biology-12-01465-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/b988ca5ccf0b/biology-12-01465-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/4fcf2c6bf286/biology-12-01465-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/6d0c143be20b/biology-12-01465-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/1fd470800255/biology-12-01465-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/273b6d034563/biology-12-01465-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/c7002d96a2f4/biology-12-01465-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9d/10740823/3897c6010e1f/biology-12-01465-g009.jpg

相似文献

1
Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1α2.Sja-Let-7 通过 Col1α2 减轻小鼠模型中四氯化碳诱导的肝纤维化。
Biology (Basel). 2023 Nov 24;12(12):1465. doi: 10.3390/biology12121465.
2
Inhibits the Growth of Hepatocellular Carcinoma Cells via Cross-Species Regulation of .通过. 的种间调控抑制肝癌细胞的生长。
Genes (Basel). 2024 Sep 4;15(9):1165. doi: 10.3390/genes15091165.
3
Sja-let-7 suppresses the development of liver fibrosis via Schistosoma japonicum extracellular vesicles.Sja-let-7通过日本血吸虫细胞外囊泡抑制肝纤维化的发展。
PLoS Pathog. 2024 Apr 10;20(4):e1012153. doi: 10.1371/journal.ppat.1012153. eCollection 2024 Apr.
4
Hepatoprotective effect of total flavonoids of Mallotus apelta (Lour.) Muell.Arg. leaf against carbon tetrachloride-induced liver fibrosis in rats via modulation of TGF-β1/Smad and NF-κB signaling pathways.马蹄麻叶总黄酮通过调控 TGF-β1/Smad 和 NF-κB 信号通路对四氯化碳诱导的大鼠肝纤维化的保护作用。
J Ethnopharmacol. 2020 May 23;254:112714. doi: 10.1016/j.jep.2020.112714. Epub 2020 Feb 24.
5
Shugan Huoxue Huayu Fang attenuates carbon tetrachloride-induced hepatic fibrosis in rats by inhibiting transforming growth factor-β1/Smad signaling.疏肝活血化瘀方通过抑制转化生长因子-β1/Smad信号通路减轻四氯化碳诱导的大鼠肝纤维化。
J Tradit Chin Med. 2022 Feb;42(1):65-72. doi: 10.19852/j.cnki.jtcm.20210624.001.
6
Physalin D attenuates hepatic stellate cell activation and liver fibrosis by blocking TGF-β/Smad and YAP signaling.岩白菜素 D 通过阻断 TGF-β/Smad 和 YAP 信号通路抑制肝星状细胞激活和肝纤维化。
Phytomedicine. 2020 Nov;78:153294. doi: 10.1016/j.phymed.2020.153294. Epub 2020 Jul 28.
7
Ligustroflavone ameliorates CCl-induced liver fibrosis through down-regulating the TGF-β/Smad signaling pathway.川芎嗪通过下调 TGF-β/Smad 信号通路改善 CCl4 诱导的肝纤维化。
Chin J Nat Med. 2021 Mar;19(3):170-180. doi: 10.1016/S1875-5364(21)60018-3.
8
Xia-yu-xue decoction (XYXD) reduces carbon tetrachloride (CCl4)-induced liver fibrosis through inhibition hepatic stellate cell activation by targeting NF-κB and TGF-β1 signaling pathways.夏雨血汤（XYXD）通过靶向NF-κB和TGF-β1信号通路抑制肝星状细胞活化，减轻四氯化碳（CCl4）诱导的肝纤维化。
BMC Complement Altern Med. 2015 Jun 30;15:201. doi: 10.1186/s12906-015-0733-1.
9
Ferulic acid attenuates liver fibrosis and hepatic stellate cell activation via inhibition of TGF-β/Smad signaling pathway.阿魏酸通过抑制TGF-β/Smad信号通路减轻肝纤维化和肝星状细胞活化。
Drug Des Devel Ther. 2018 Dec 3;12:4107-4115. doi: 10.2147/DDDT.S186726. eCollection 2018.
10
A schistosome miRNA promotes host hepatic fibrosis by targeting transforming growth factor beta receptor III.一种血吸虫微小 RNA 通过靶向转化生长因子-β受体 III 促进宿主肝纤维化。
J Hepatol. 2020 Mar;72(3):519-527. doi: 10.1016/j.jhep.2019.10.029. Epub 2019 Nov 16.

引用本文的文献

1
Roles of helminth extracellular vesicle-derived let-7 in host-parasite crosstalk.寄生虫细胞外囊泡衍生的 let-7 在宿主-寄生虫串扰中的作用。
Front Immunol. 2024 Oct 28;15:1449495. doi: 10.3389/fimmu.2024.1449495. eCollection 2024.
2
Inhibits the Growth of Hepatocellular Carcinoma Cells via Cross-Species Regulation of .通过. 的种间调控抑制肝癌细胞的生长。
Genes (Basel). 2024 Sep 4;15(9):1165. doi: 10.3390/genes15091165.

本文引用的文献

1
Global profiling of miRNA and protein expression patterns in rabbit peritoneal macrophages treated with exosomes derived from Taenia pisiformis cysticercus.从猪囊尾蚴衍生的 exosomes 处理后的兔腹膜巨噬细胞中 miRNA 和蛋白质表达谱的全局分析。
Genomics. 2023 Sep;115(5):110690. doi: 10.1016/j.ygeno.2023.110690. Epub 2023 Jul 23.
2
A Novel miRNA From Egg-Derived Exosomes of Promotes Liver Fibrosis in Murine Schistosomiasis.一种新型 miRNA 来自卵源性外泌体，可促进小鼠日本血吸虫病肝纤维化。
Front Immunol. 2022 Apr 27;13:860807. doi: 10.3389/fimmu.2022.860807. eCollection 2022.
3
From Inflammation to Fibrosis: Novel Insights into the Roles of High Mobility Group Protein Box 1 in Schistosome-Induced Liver Damage.
从炎症到纤维化：对高迁移率族蛋白盒1在血吸虫诱导的肝损伤中作用的新见解。
Pathogens. 2022 Feb 24;11(3):289. doi: 10.3390/pathogens11030289.
4
Identification of a MicroRNA That Suppresses Hepatoma Cell Growth and Migration by Targeting Host Gene.鉴定通过靶向宿主基因抑制肝癌细胞生长和迁移的 microRNA。
Front Cell Infect Microbiol. 2022 Jan 31;12:786543. doi: 10.3389/fcimb.2022.786543. eCollection 2022.
5
Schistosome immunomodulators.血吸虫免疫调节剂。
PLoS Pathog. 2021 Dec 30;17(12):e1010064. doi: 10.1371/journal.ppat.1010064. eCollection 2021 Dec.
6
rSjP40 Inhibited the Activity of Collagen Type I Promoter Ets-1 in HSCs.rSjP40抑制肝星状细胞中I型胶原启动子Ets-1的活性。
Front Cell Dev Biol. 2021 Nov 8;9:765616. doi: 10.3389/fcell.2021.765616. eCollection 2021.
7
Exosomal microRNA let-7-5p from Cysticercus Prompted Macrophage to M2 Polarization through Inhibiting the Expression of C/EBP δ.来自猪带绦虫囊尾蚴的外泌体微小RNA let-7-5p通过抑制C/EBP δ的表达促使巨噬细胞向M2极化。
Microorganisms. 2021 Jun 29;9(7):1403. doi: 10.3390/microorganisms9071403.
8
The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.rSjP40 通过抑制 let-7b 抑制肝星状细胞活化的作用。
PLoS Negl Trop Dis. 2021 Jun 23;15(6):e0009472. doi: 10.1371/journal.pntd.0009472. eCollection 2021 Jun.
9
Schistosome Infection and Schistosome-Derived Products as Modulators for the Prevention and Alleviation of Immunological Disorders.血吸虫感染和血吸虫衍生产物作为免疫性疾病预防和缓解的调节剂。
Front Immunol. 2021 Feb 22;12:619776. doi: 10.3389/fimmu.2021.619776. eCollection 2021.
10
The Differential and Dynamic Progression of Hepatic Inflammation and Immune Responses During Liver Fibrosis Induced by or Carbon Tetrachloride in Mice.或四氯化碳诱导的小鼠肝纤维化过程中肝脏炎症和免疫反应的差异和动态进展。
Front Immunol. 2020 Oct 7;11:570524. doi: 10.3389/fimmu.2020.570524. eCollection 2020.