Zaharioudakis Konstantinos, Kollia Eleni, Leontiou Areti, Moschovas Dimitrios, Karydis-Messinis Andreas, Avgeropoulos Apostolos, Zafeiropoulos Nikolaos E, Ragkava Efthymia, Kehayias George, Proestos Charalampos, Salmas Constantinos E, Giannakas Aris E
Department of Food Science and Technology, University of Patras, 30100 Agrinio, Greece.
Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens Zografou, 15771 Athens, Greece.
Nanomaterials (Basel). 2023 Dec 18;13(24):3161. doi: 10.3390/nano13243161.
Carvacrol is well documented for its antibacterial and antioxidant effects. However, its high volatility has directed researchers toward nanoencapsulation technology according to bioeconomy and sustainability trends. This study examined and compared free carvacrol (FC), carvacrol microemulsion (MC), carvacrol microemulsion busted with chitosan (MMC), and carvacrol nanoemulsions (NC) as active coatings on extending minced pork meat shelf life at 4 ± 1 °C for 9 days, focusing on microbiological, physiochemical, and sensory characteristics. The research involved pre-characterizing droplet sizes, evaluating antioxidants, and determining antibacterial efficacy. The results demonstrated that NC with a 21 nm droplet size exhibited the highest antioxidant and antibacterial activity. All coatings succeeded in extending the preservation of fresh minced pork meat in comparison to the free carvacrol sample (FC). The NC coating showed the highest extension of minced pork meat preservation and maintained meat freshness for 9 days, with a lower TBARs of 0.736 mg MDA/Kg, and effectively reduced mesophilic, lactic acid, and psychotrophic bacterial counts more significantly by 1.2, 2, and 1.3 log, respectively, as compared to FC. Sensory assessments confirmed the acceptability of NC and MCC coatings. Overall, the carvacrol-based nanoemulsion can be considered a novel antioxidant and antimicrobial active coating due to its demonstrated higher efficacy in all the examined tests performed.
香芹酚的抗菌和抗氧化作用已有充分文献记载。然而,根据生物经济和可持续发展趋势,其高挥发性促使研究人员转向纳米包封技术。本研究考察并比较了游离香芹酚(FC)、香芹酚微乳液(MC)、壳聚糖破乳香芹酚微乳液(MMC)和香芹酚纳米乳液(NC)作为活性涂层在4±1℃下延长绞碎猪肉保质期9天的效果,重点关注微生物学、理化和感官特性。该研究包括对液滴尺寸进行预表征、评估抗氧化剂以及测定抗菌效果。结果表明,液滴尺寸为21nm的NC表现出最高的抗氧化和抗菌活性。与游离香芹酚样品(FC)相比,所有涂层都成功延长了新鲜绞碎猪肉的保存期。NC涂层显示出绞碎猪肉保存期的最大延长,并在9天内保持了肉的新鲜度,硫代巴比妥酸反应物(TBARs)较低,为0.736mg丙二醛/千克,与FC相比,分别有效降低嗜温菌、乳酸菌和嗜冷菌数量1.2、2和1.3个对数级。感官评估证实了NC和MMC涂层的可接受性。总体而言,基于香芹酚的纳米乳液因其在所有检测试验中表现出更高的功效,可被视为一种新型的抗氧化和抗菌活性涂层。