Mandeville R, Pateisky N, Philipp K, Kubista E, Dumas F, Grouix B
Anticancer Res. 1986 Nov-Dec;6(6):1257-63.
The results of preliminary investigations into the immunolymphscintigraphic (ILS) detection of axillary lymph node metastases in nine breast cancer patients by means of the 3C6F9 monoclonal antibody (MAs) are presented. The IgG2a monoclonal antibody detects a 37 KD antigen, consistently found on the surface of primary and metastatic breast tumors. Each patient received 1 mCi of I-123 (specific activity, 2 mCi per mg of antibody) as a subcutaneous injection between the 2nd and the 3rd finger of both hands, i.e., the healthy side serving as a control for the affected side. Clear images of lymph node metastases were visible 4 to 8 hours after injection of the antibody. Seven of the nine patients studied were positive by scanning and six showed positive lymph node involvement by histopathology (6/7; true positive = 86%). Two patients did not show any iodine uptake in the axilla and were subsequently found to be free of metastases (2/2; true negative = 100%). These data give an overall accuracy of ILS of 89% and demonstrate that 3C6F9 localizes preferentially in affected axillary lymph nodes compared to normal lymph nodes.
本文展示了对9例乳腺癌患者采用3C6F9单克隆抗体（MAs）进行免疫淋巴闪烁造影（ILS）检测腋窝淋巴结转移的初步研究结果。IgG2a单克隆抗体可检测到一种37KD抗原，该抗原始终存在于原发性和转移性乳腺肿瘤表面。每位患者在双手的第二和第三指之间皮下注射1mCi的I-123（比活度为每毫克抗体2mCi），即以健康侧作为患侧的对照。注射抗体后4至8小时可见清晰的淋巴结转移图像。研究的9例患者中有7例扫描呈阳性，6例经组织病理学检查显示有阳性淋巴结受累（6/7；真阳性=86%）。2例患者腋窝未显示任何碘摄取，随后发现无转移（2/2；真阴性=100%）。这些数据表明ILS的总体准确率为89%，并证明与正常淋巴结相比，3C6F9优先定位于受影响的腋窝淋巴结。
