Yu Jun-Dong, Wang Jing-Yi, Liu Jia-Yuan, Xu Ye-Chun, Zhang Xue, Du Jun, Wu Xiao-Hong, Chen Liang, Wu Ying-Chun, Li Yi-Ming
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
Fitoterapia. 2024 Mar;173:105780. doi: 10.1016/j.fitote.2023.105780. Epub 2023 Dec 20.
In this study, 16 new ent-labdane-type diterpene glycosides, designated as goshonosides J1-J16 (1-16), along with nine previously known diterpene glycosides (17-25) were extracted from the fruits of Rubus chingii Hu. The structures of goshonosides J1-J16 were elucidated using various analytical techniques, such as nuclear magnetic resonance, electron capture detector ECD, high-resolution electrospray ionization mass spectrometry HREIMS, single-crystal X-ray diffraction, and hydrolysis. Furthermore, the isolates' efficacy in inhibiting the activity of phosphodiesterase type 5 A was evaluated. Goshonosides J1, J2, and G effectively inhibited the activity of the aforementioned enzyme (IC values: 6.15 ± 1.76, 3.27 ± 0.65, and 9.61 ± 2.36 μM, respectively). Our findings highlight the remarkable structural diversity of bioactive compounds in R. chingii Hu and offer insights into the use of this shrub.
在本研究中，从掌叶覆盆子果实中提取出16种新的对映-贝壳杉烷型二萜糖苷，命名为高野苷J1-J16（1-16），以及9种先前已知的二萜糖苷（17-25）。利用各种分析技术，如核磁共振、电子捕获检测器（ECD）、高分辨率电喷雾电离质谱（HREIMS）、单晶X射线衍射和水解等，阐明了高野苷J1-J16的结构。此外，还评估了分离物对5A型磷酸二酯酶活性的抑制效果。高野苷J1、J2和G能有效抑制上述酶的活性（IC值分别为6.15±1.76、3.27±0.65和9.61±2.36μM）。我们的研究结果突出了掌叶覆盆子中生物活性化合物显著的结构多样性，并为该灌木的利用提供了见解。
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