Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Chemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Sci Rep. 2023 Dec 22;13(1):22940. doi: 10.1038/s41598-023-49637-6.
Nanotechnology holds significant promise for the development of novel and necessary products that enhance human health. Pharmacology and nanotechnology have contributed to developing advanced and highly effective drugs for cancer treatment and combating microbial infections. The microbiological effectiveness against the variety of examined microorganisms was assessed using the time killer curve, scanning electron microscopy (SEM), MIC techniques, and the agar well diffusion method. SEM was utilized to enhance the analysis of the mechanisms underlying the bio-interface interaction and intracellular localization of calcium oxide nanoparticles (CaONPs). The MTT test was used to examine the cytotoxicity of CaONP anticancer activity in various cancer cells, including colon, breast, and hepatic cells. The efficacy of CaONPs as an anticancer medication was elucidated by analyzing the gene expression of both treated and untreated cancer cells. MIC and MBC of CaONPs against Escherichia coli and Staphylococcus epidermidis were 150, 150, 150, and 200 µg/ml, respectively. The MIC and MFC of CaONPs against Candida albicans were 200 µg/ml and 250 µg/ml, respectively. The IC50 values of various CaONPs vary depending on the type of cancer cells. The gene expression analysis of breast cancer cells undergoing treatment revealed the identification of several cancer-controlling genes, namely BAX, BCL2, P53, TERT, KRAS1, KRAS2, and RB1. The study demonstrated the notable antibacterial efficacy of CaONPs, highlighting their potential as cancer therapies.
纳米技术在开发新型和必要的产品方面具有重要的应用前景，这些产品可以增强人类健康。药理学和纳米技术为开发用于癌症治疗和抗击微生物感染的先进、高效药物做出了贡献。使用时间杀伤曲线、扫描电子显微镜 (SEM)、微量稀释法 (MIC) 技术和琼脂孔扩散法评估了对各种检查微生物的微生物学效果。SEM 用于增强对生物界面相互作用和氧化钙纳米颗粒 (CaONPs) 细胞内定位机制的分析。MTT 试验用于研究 CaONP 在各种癌细胞（包括结肠、乳腺和肝细胞）中的抗癌活性的细胞毒性。通过分析经处理和未经处理的癌细胞的基因表达，阐明了 CaONP 作为抗癌药物的疗效。CaONP 对大肠杆菌和表皮葡萄球菌的 MIC 和 MBC 分别为 150、150、150 和 200μg/ml。CaONP 对白色念珠菌的 MIC 和 MFC 分别为 200μg/ml 和 250μg/ml。不同 CaONPs 的 IC50 值取决于癌细胞的类型。经过治疗的乳腺癌细胞的基因表达分析表明，鉴定出了几种控制癌症的基因，即 BAX、BCL2、P53、TERT、KRAS1、KRAS2 和 RB1。该研究表明 CaONPs 具有显著的抗菌功效，突出了其作为癌症治疗方法的潜力。
