Shahid Mohammad, Saeed Nermin Kamal, Ahmad Nayeem, Shadab Mohd, Joji Ronni Mol, Al-Mahmeed Ali, Bindayna Khalid M, Tabbara Khaled Saeed, Ismaeel Abdulrahman Y, Dar Fazal K
Department of Microbiology, Immunology, and Infectious Diseases, College of Medicine & Medical Sciences, Arabian Gulf University, Manama 329, Bahrain.
Microbiology Section, Department of Pathology, Salmaniya Medical Complex, Manama 435, Bahrain.
J Clin Med. 2023 Dec 5;12(24):7522. doi: 10.3390/jcm12247522.
The emergence of extended-spectrum β-lactamase-producing , including CRKP infections, has resulted in significant morbidity and mortality worldwide. We aimed to explore the presence of genes (CTX-M, TEM, and SHV) in CRKP isolates. A total of 24 CRKP isolates were randomly selected from the Salmaniya Medical Complex Microbiology Laboratory. These isolates, which were positive for carbapenemases, were further explored for CTX-M, TEM, and SHV genes using PCR. All the CTX-M PCR amplicons were sent for sequencing. To determine genetic relatedness, molecular typing by ERIC-PCR was performed. The gene testing demonstrated that a significant proportion of these isolates harbored SHV, CTX-M, and TEM genes (100%, 91.6%, and 45.8%), respectively. Bioinformatic analyses confirmed CTX-M-15 in these isolates. ERIC-PCR analysis showed three clusters demonstrating genetic relatedness. The study findings reveal the concomitant carriage of the SHV and CTX-M-15 and a comparatively lower carriage of TEM genes in CRKP isolates. Our findings highlight the significance of routinely reporting the presence of antibiotic resistance genes along with regular antibiotic sensitivity reports, as this will aid clinicians in prescribing appropriate antibiotics.
产超广谱β-内酰胺酶（包括耐碳青霉烯类肺炎克雷伯菌感染）的出现已在全球范围内导致了显著的发病率和死亡率。我们旨在探究耐碳青霉烯类肺炎克雷伯菌分离株中（CTX-M、TEM和SHV）基因的存在情况。从萨勒曼尼亚医疗中心微生物实验室随机选取了24株耐碳青霉烯类肺炎克雷伯菌分离株。这些对碳青霉烯酶呈阳性的分离株，使用聚合酶链反应（PCR）进一步探究CTX-M、TEM和SHV基因。所有CTX-M PCR扩增产物均送去测序。为确定遗传相关性，进行了肠杆菌基因间重复一致序列聚合酶链反应（ERIC-PCR）分子分型。基因检测表明，这些分离株中有很大比例分别携带SHV、CTX-M和TEM基因（分别为100%、91.6%和45.8%）。生物信息学分析证实这些分离株中存在CTX-M-15。ERIC-PCR分析显示三个聚类表明存在遗传相关性。研究结果揭示了耐碳青霉烯类肺炎克雷伯菌分离株中SHV和CTX-M-15的共同携带以及TEM基因相对较低的携带率。我们的研究结果强调了在常规抗生素敏感性报告的同时常规报告抗生素耐药基因存在情况的重要性，因为这将有助于临床医生开具合适的抗生素。
