Vasiliauskienė Dovilė, Lukša Juliana, Servienė Elena, Urbonavičius Jaunius
Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University (VILNIUS TECH), Saulėtekio al. 11, 10223 Vilnius, Lithuania.
Life (Basel). 2023 Dec 7;13(12):2306. doi: 10.3390/life13122306.
In today's world, the use of environmentally friendly materials is strongly encouraged. These materials derive from primary raw materials of plant origin, like fibrous hemp, flax, and bamboo, or recycled materials, such as textiles or residual paper, making them suitable for the growth of microorganisms. Here, we investigate changes in bacterial communities in biocomposites made of hemp shives, corn starch, and either expandable graphite or a Flovan compound as flame retardants. Using Next Generation Sequencing (NGS), we found that after 12 months of incubation at 22 °C with a relative humidity of 65%, Proteobacteria accounted for >99.7% of the microbiome in composites with either flame retardant. By contrast, in the absence of flame retardants, the abundance of Proteobacteria decreased to 32.1%, while Bacteroidetes (36.6%), Actinobacteria (8.4%), and Saccharobacteria (TM7, 14.51%) appeared. Using the increasing concentrations of either expandable graphite or a Flovan compound in an LB medium, we were able to achieve up to a 5-log reduction in the viability of , , representatives of the and genera, the abundance of which varied in the biocomposites tested. Our results demonstrate that flame retardants act on both Gram-positive and Gram-negative bacteria and suggest that their antimicrobial activities also have to be tested when producing new compounds.
在当今世界，大力提倡使用环保材料。这些材料来源于植物源的主要原材料，如纤维大麻、亚麻和竹子，或回收材料，如纺织品或废纸，使其适合微生物生长。在此，我们研究了由大麻屑、玉米淀粉以及可膨胀石墨或弗洛万化合物作为阻燃剂制成的生物复合材料中细菌群落的变化。使用下一代测序（NGS）技术，我们发现，在22°C、相对湿度65%的条件下孵育12个月后，两种含阻燃剂的复合材料中，变形菌门在微生物群落中所占比例均超过99.7%。相比之下，在没有阻燃剂的情况下，变形菌门的丰度降至32.1%，而拟杆菌门（36.6%）、放线菌门（8.4%）和糖杆菌门（TM7，14.51%）出现。在LB培养基中使用浓度不断增加的可膨胀石墨或弗洛万化合物，我们能够使肠杆菌属、芽孢杆菌属和葡萄球菌属的代表菌的活力降低多达5个对数，这些菌属在测试的生物复合材料中的丰度各不相同。我们的结果表明，阻燃剂对革兰氏阳性菌和革兰氏阴性菌均有作用，并表明在生产新化合物时也必须测试其抗菌活性。
