Ekeuku Sophia Ogechi, Tan Jen-Kit, Al-Saadi Hiba Murtadha, Ahmad Fairus, Elvy Suhana Mohd Ramli, Arlamsyah Azlan Mohd, Japar Sidik Fadhlullah Zuhair, Abdul Hamid Juliana, Ima-Nirwana Soelaiman, Chin Kok-Yong
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Malaysia.
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, Malaysia.
Life (Basel). 2023 Dec 15;13(12):2343. doi: 10.3390/life13122343.
Osteoarthritis (OA) is a degenerative joint condition with limited disease-modifying treatments currently. Palm tocotrienol-rich fraction (TRF) has been previously shown to be effective against OA, but its mechanism of action remains elusive. This study aims to compare serum metabolomic alteration in Sprague-Dawley rats with monosodium iodoacetate (MIA)-induced OA which were treated with palm TRF, glucosamine sulphate, or a combination of both. This study was performed on thirty adult male rats, which were divided into normal control ( = 6) and OA groups ( = 24). The OA group received intra-articular injections of MIA and daily oral treatments of refined olive oil (vehicle, = 6), palm TRF (100 mg/kg, = 6), glucosamine sulphate (250 mg/kg, = 6), or a combination of TRF and glucosamine ( = 6) for four weeks. Serum was collected at the study's conclusion for metabolomic analysis. The findings revealed that MIA-induced OA influences amino acid metabolism, leading to changes in metabolites associated with the biosynthesis of phenylalanine, tyrosine and tryptophan as well as alterations in the metabolism of phenylalanine, tryptophan, arginine and proline. Supplementation with glucosamine sulphate, TRF, or both effectively reversed these metabolic changes induced by OA. The amelioration of metabolic effects induced by OA is linked to the therapeutic effects of TRF and glucosamine. However, it remains unclear whether these effects are direct or indirect in nature.
骨关节炎（OA）是一种退行性关节疾病，目前疾病改善治疗方法有限。棕榈富含生育三烯酚组分（TRF）此前已被证明对OA有效，但其作用机制仍不清楚。本研究旨在比较用棕榈TRF、硫酸氨基葡萄糖或两者联合治疗的碘乙酸钠（MIA）诱导的OA的Sprague-Dawley大鼠的血清代谢组学变化。本研究对30只成年雄性大鼠进行，分为正常对照组（n = 6）和OA组（n = 24）。OA组接受关节内注射MIA，并每日口服精制橄榄油（载体，n = 6）、棕榈TRF（100 mg/kg，n = 6）、硫酸氨基葡萄糖（250 mg/kg，n = 6）或TRF与氨基葡萄糖的组合（n = 6），持续四周。在研究结束时收集血清进行代谢组学分析。研究结果显示，MIA诱导的OA影响氨基酸代谢，导致与苯丙氨酸、酪氨酸和色氨酸生物合成相关的代谢物变化以及苯丙氨酸、色氨酸、精氨酸和脯氨酸代谢的改变。补充硫酸氨基葡萄糖、TRF或两者可有效逆转OA诱导的这些代谢变化。OA诱导的代谢效应的改善与TRF和氨基葡萄糖的治疗效果有关。然而，这些效应本质上是直接的还是间接的仍不清楚。
