Effects of Exercise on Gut Microbiome and Serum Metabolomics in Post-Traumatic Osteoarthritis Rats.

The aim of this work is to investigate the impact of exercise on gut microbiome composition, serum metabolites, and their correlation with osteoarthritis (OA) severity. Thirty-six Sprague-Dawley (SD) rats were randomly divided into four groups: Sham rats without treadmill walking (Sham/Sed group, = 9), Sham rats with treadmill walking 2 months (Sham/TW2M group, = 9), PTOA rats without treadmill walking (PTOA/Sed group, = 9), and PTOA rats with treadmill walking 2 months (PTOA/TW2M group, = 9). The PTOA model was induced by transection of the anterior cruciate ligament (ACLT) and destabilization of the medial meniscus (DMM). Histological evaluation and micro-CT analysis were performed to observe the pathological changes in cartilage and subchondral bone, respectively. Additionally, we conducted 16S rDNA sequencing of fecal samples and untargeted metabolomic analysis using liquid chromatography-mass spectrometry (LC-MS) of serum samples to detect the alteration of gut microbiota composition and metabolites. Exercise effectively mitigated OA-related pathological changes, including articular cartilage degeneration and subchondral bone loss. Moreover, 16S rDNA sequencing analysis of gut microbiome revealed a decreased abundance of ( < 0.01), ( < 0.01), ( < 0.01), [] ( < 0.01), and ( < 0.01) but an increase in ( < 0.01) in PTOA/TW2M group rats compared with PTOA/Sed group as a response to exercise. In addition, the results of metabolomics analysis showed that exercise treatment contributed to the upregulation of Daidzein and Anthranilic acid and downregulation of 1-Palmitoyllysophosphatidylcholine. Moreover, the correlation analysis showed that significantly positively correlated with both OARSI (r = 0.81, < 0.01) and Mankin score (r = 0.83, < 0.01) and negatively correlated with the serum level of Anthranilic acid (r = -0.56, < 0.01) and Daidzein (r = -0.46, < 0.01). Exercise can effectively mitigate OA through slowing down articular cartilage degeneration and subchondral bone loss, modulating gut microbiota composition, and increasing beneficial metabolites.