Zhao Xuli, Meng Tianzhu, Jin Shenghan, Ren Kaixing, Cai Zhe, Cai Bo, Li Saibao
College of Agricultural Science and Engineering, Hohai University, No. 8 Focheng West Road, Nanjing 211100, China.
College of Water Resources and Civil Engineering, Tibet Agricultural and Animal Husbandry University, No. 8 Xueyuan Road, Linzhi 860000, China.
Microorganisms. 2023 Nov 21;11(12):2829. doi: 10.3390/microorganisms11122829.
Quinoa is extensively cultivated for its nutritional value, and its exceptional capacity to endure elevated salt levels presents a promising resolution to the agricultural quandaries posed by salinity stress. However, limited research has been dedicated to elucidating the correlation between alterations in the salinity soil microbial community and nitrogen transformations. To scrutinize the underlying mechanisms behind quinoa's salt tolerance, we assessed the changes in microbial community structure and the abundance of nitrogen transformation genes across three distinct salinity thresholds (1 g·kg, 3 g·kg, and 6 g·kg) at two distinct time points (35 and 70 days). The results showed the positive effect of quinoa on the soil microbial community structure, including changes in key populations and its regulatory role in soil nitrogen cycling under salt stress. , , and were inhibited by increased salinity, while the relative abundance of increased. and showed relatively stable abundances across time and salinity levels. Quinoa possesses the ability to synthesize or modify the composition of keystone species or promote the establishment of highly complex microbial networks (modularity index > 0.4) to cope with fluctuations in external salt stress environments. Furthermore, quinoa exhibited nitrogen (N) cycling by downregulating denitrification genes (, ), upregulating nitrification genes (Archaeal (AOA), Bacterial (AOB)), and stabilizing nitrogen fixation genes () to absorb nitrate-nitrogen (NO_N). This study paves the way for future research on regulating quinoa, promoting soil microbial communities, and nitrogen transformation in saline environments.
藜麦因其营养价值而被广泛种植，其耐受高盐水平的特殊能力为盐胁迫带来的农业难题提供了一个有前景的解决方案。然而，致力于阐明盐渍土壤微生物群落变化与氮转化之间相关性的研究有限。为了探究藜麦耐盐性的潜在机制，我们在两个不同时间点（35天和70天）评估了三种不同盐度阈值（1 g·kg、3 g·kg和6 g·kg）下微生物群落结构的变化以及氮转化基因的丰度。结果表明藜麦对土壤微生物群落结构有积极影响，包括关键种群的变化及其在盐胁迫下对土壤氮循环的调节作用。 、 和 受到盐度增加的抑制，而 的相对丰度增加。 和 在不同时间和盐度水平下表现出相对稳定的丰度。藜麦具有合成或改变关键物种组成或促进高度复杂微生物网络（模块化指数>0.4）建立的能力，以应对外部盐胁迫环境的波动。此外，藜麦通过下调反硝化基因（ 、 ）、上调硝化基因（古菌 （AOA）、细菌 （AOB））和稳定固氮基因（ ）来吸收硝态氮（NO_N），从而实现氮（N）循环。本研究为未来调控藜麦、促进土壤微生物群落以及盐渍环境中氮转化的研究铺平了道路。
