Ramírez Castillo Flor Yazmín, Guerrero Barrera Alma Lilian, Harel Josée, Avelar González Francisco Javier, Vogeleer Philippe, Arreola Guerra José Manuel, González Gámez Mario
Laboratorio de Biología Celular y Tisular, Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes 20100, Mexico.
Département de Pathologie et de Microbiologie, Centre de Recherche en Infectologie Porcine et Avicole, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 7C6, Canada.
Microorganisms. 2023 Nov 25;11(12):2858. doi: 10.3390/microorganisms11122858.
Uropathogenic (UPEC) strains are among the leading causes of urinary tract infections (UTIs) worldwide. They can colonize the urinary tract and form biofilms that allow bacteria to survive and persist, causing relapses of infections and life-threatening sequelae. Here, we analyzed biofilm production, antimicrobial susceptibility, virulence factors, and phylogenetic groups in 74 isolated from diagnosed patients with UTIs to describe their microbiological features and ascertain their relationship with biofilm capabilities. High levels of ceftazidime resistance are present in hospital-acquired UTIs. Isolates of multidrug resistance strains ( 0.0017) and the gene ( = 0.0193) were higher in male patients. All the strains tested were able to form biofilms. Significant differences were found among higher optical densities (ODs) and antibiotic resistance to cefazolin ( = 0.0395), ceftazidime ( = 0.0302), and cefepime ( = 0.0420). Overall, the presence of and coincided with strong biofilm formation by UPEC. Type 1 fimbriae ( = 0.0349), curli ( = 0.0477), and cellulose ( = 0.0253) production was significantly higher among strong biofilm formation. Our results indicated that high antibiotic resistance may be related to male infections as well as strong and moderate biofilm production. The ability of strains to produce biofilm is important for controlling urinary tract infections.
尿路致病性大肠杆菌（UPEC）菌株是全球尿路感染（UTIs）的主要病因之一。它们能够在尿路中定殖并形成生物膜，使细菌得以存活和持续存在，导致感染复发和危及生命的后遗症。在此，我们分析了从UTIs确诊患者中分离出的74株菌株的生物膜形成、抗菌药敏性、毒力因子和系统发育群，以描述它们的微生物学特征，并确定它们与生物膜形成能力的关系。医院获得性UTIs中存在高水平的头孢他啶耐药性。男性患者中多重耐药菌株（0.0017）和某基因（=0.0193）的分离株更高。所有测试菌株都能够形成生物膜。在较高的光密度（ODs）以及对头孢唑林（=0.0395）、头孢他啶（=0.0302）和头孢吡肟（=0.0420）的抗生素耐药性之间发现了显著差异。总体而言，某物质和另一物质的存在与UPEC形成强烈生物膜相吻合。在强烈生物膜形成过程中，1型菌毛（=0.0349）、卷曲菌毛（=0.0477）和纤维素（=0.0253）的产生显著更高。我们的结果表明，高抗生素耐药性可能与男性感染以及强烈和中度生物膜形成有关。大肠杆菌菌株产生生物膜的能力对于控制尿路感染很重要。
