Käbisch Lisa, Schink Anne-Kathrin, Hoeltig Doris, Verspohl Jutta, Gyuranecz Miklós, Spergser Joachim, Kehrenberg Corinna, Schwarz Stefan
Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Microorganisms. 2023 Nov 29;11(12):2881. doi: 10.3390/microorganisms11122881.
Organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee of Antimicrobial Susceptibility Testing (EUCAST) provide standardized methodologies for antimicrobial susceptibility testing of a wide range of nonfastidious and fastidious bacteria, but so far not for spp. of animal origin. Recently, a proposed method for the standardized broth microdilution testing of using commercial Sensititre microtiter plates was presented. In this study, we evaluated this broth microdilution method with 37 field isolates and tested their susceptibility toward the following antimicrobial agents: doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin. The isolates originated from different countries, isolation sites, and years. The broth microdilution method was carried out using a modified Friis broth as the culture and test medium. For macrolides and lincosamides, a bimodal distribution with elevated MIC values could be observed for almost half of the tested field isolates, deducing reduced susceptibility toward these substances. With a recently published protocol, we were able to test a variety of field isolates, and consistent data could be obtained. Using this method, monitoring studies of isolates can be carried out in a comparable manner, and the observed susceptibility profiles can be screened for possible changes in MIC values in the future.
像临床和实验室标准协会（CLSI）或欧洲抗菌药物敏感性试验委员会（EUCAST）这样的组织提供了针对多种非苛养菌和苛养菌进行抗菌药物敏感性试验的标准化方法，但到目前为止，尚未针对动物源的[具体菌种名称未给出]提供此类方法。最近，有人提出了一种使用商业Sensititre微量滴定板对[具体菌种名称未给出]进行标准化肉汤微量稀释试验的方法。在本研究中，我们用37株田间分离株评估了这种肉汤微量稀释法，并测试了它们对以下抗菌药物的敏感性：强力霉素、恩诺沙星、红霉素、氟苯尼考、庆大霉素、马波沙星、四环素、泰妙菌素、替米考星、泰拉霉素和泰乐菌素。这些分离株来自不同的国家、分离地点和年份。肉汤微量稀释法采用改良的弗里斯肉汤作为培养和测试培养基进行。对于大环内酯类和林可酰胺类药物，几乎一半的受试田间分离株观察到MIC值升高的双峰分布，表明对这些物质的敏感性降低。通过最近公布的方案，我们能够测试多种田间分离株，并获得一致的数据。使用这种方法，可以以可比的方式开展对[具体菌种名称未给出]分离株的监测研究，并且可以筛选观察到的敏感性谱，以了解未来MIC值可能发生的变化。
