急性运动可迅速使糖尿病大鼠心肌肌球蛋白重链同工型 mRNA 组成正常化：对糖尿病心肌病的影响。
Acute Exercise Promptly Normalizes Myocardial Myosin Heavy-Chain Isoform mRNA Composition in Diabetic Rats: Implications for Diabetic Cardiomyopathy.
机构信息
Exercise Science Department, Yarmouk University, Irbid 21163, Jordan.
Department of Biological Sciences, Yarmouk University, Irbid 21163, Jordan.
出版信息
Medicina (Kaunas). 2023 Dec 18;59(12):2193. doi: 10.3390/medicina59122193.
The acute effects of exercise on the myosin heavy-chain (MHC) isoform mRNA expression and the upstream transcription factors in diabetic and non-diabetic hearts remain unexplored. We aimed to determine the acute effect of a single exercise session on the expression of left ventricular MHC, MHC-α and MHC-β, and thyroid receptor (TR), TR-α1 and TR-β, isoform mRNA in diabetic and non-diabetic rats. Sprague-Dawley rats were assigned to four groups: non-diabetic control (CS), diabetic exercise (DIEX), sedentary diabetic (DIS), and non-diabetic exercise (CEX). Diabetes was induced via streptozotocin injection (55 mg/kg). DIEX and CEX rats performed an exercise session (60 min at 50 m/min and 0% grade) 6-7 weeks after diabetes induction. MHC-α mRNA was lower in DIS ( = 0.03) and not different in DIEX ( = 0.1) relative to CS. DIS showed higher MHC-β mRNA than the non-diabetic rats, CS and CEX ( = 0.02 and = 0.009, respectively). MHC-β mRNA in DIEX was normalized to non-diabetic levels in CS ( = 0.3). TR-α1 was higher in DIS and not different in DIEX relative to CS and CEX ( = 0.03 and = 1.0, respectively). In CEX, exercise did not change MHC-α, MHC-β, and TR-α1 relative to CS ( = 1.0). TR-β was not different between groups. In conclusion, exercise appears to acutely normalize the myocardial MHC and TR isoform mRNA expression only in the diabetic heart. These responses may induce therapeutic mechanisms other than changing the MHC isoform composition.
运动对糖尿病和非糖尿病心脏肌球蛋白重链（MHC）同工型 mRNA 表达和上游转录因子的急性影响仍不清楚。我们旨在确定单次运动对糖尿病和非糖尿病大鼠左心室 MHC、MHC-α 和 MHC-β 以及甲状腺受体（TR）、TR-α1 和 TR-β 同工型 mRNA 表达的急性影响。Sprague-Dawley 大鼠分为四组：非糖尿病对照组（CS）、糖尿病运动组（DIEX）、久坐糖尿病组（DIS）和非糖尿病运动组（CEX）。糖尿病通过链脲佐菌素注射（55mg/kg）诱导。DIEX 和 CEX 大鼠在糖尿病诱导后 6-7 周进行运动（50m/min 和 0%坡度下 60 分钟）。DIS 的 MHC-α mRNA 低于 CS（ = 0.03），与 DIEX 无差异（ = 0.1）。DIS 的 MHC-β mRNA 高于非糖尿病大鼠 CS 和 CEX（ = 0.02 和 = 0.009）。DIEX 的 MHC-β mRNA 与 CS 的非糖尿病水平正常化（ = 0.3）。与 CS 和 CEX 相比，DIS 的 TR-α1 更高，而 DIEX 无差异（ = 0.03 和 = 1.0）。在 CEX 中，与 CS 相比，运动没有改变 MHC-α、MHC-β 和 TR-α1（ = 1.0）。TR-β 在各组之间没有差异。总之，运动似乎仅在糖尿病心脏中急性使心肌 MHC 和 TR 同工型 mRNA 表达正常化。这些反应可能会诱导除改变 MHC 同工型组成之外的治疗机制。
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