Department of Medicine, Faculty of Medicine, University Complutense, 28040 Madrid, Spain.
GINTRAMIS Research Group (Translational Research Group on Microbiota and Health), Faculty of Medicine, University Complutense, 28040 Madrid, Spain.
Nutrients. 2023 Dec 12;15(24):5077. doi: 10.3390/nu15245077.
Scientific evidence has increasingly supported the beneficial effects of probiotic-based food supplements on human intestinal health. This ex vivo study investigated the effects on the composition and metabolic activity of the intestinal microbiota of three probiotic-based food supplements, containing, respectively, (1) ES1, (2) NCFM, and (3) a combination of NCFM, Lpc-37™, Bi-07™, and Bl-04™. This study employed fecal samples from six healthy donors, inoculated in a Colon-on-a-plate system. After 48 h of exposure or non-exposure to the food supplements, the effects were measured on the overall microbial fermentation (pH), changes in microbial metabolic activity through the production of short-chain and branched-chain fatty acids (SCFAs and BCFAs), ammonium, lactate, and microbial composition. The strongest effect on the fermentation process was observed for the combined formulation probiotics, characterized by the significant stimulation of butyrate production, a significant reduction in BCFAs and ammonium in all donors, and a significant stimulatory effect on bifidobacteria and lactobacilli growth. Our findings suggest that the combined formulation probiotics significantly impact the intestinal microbiome of the healthy individuals, showing changes in metabolic activity and microbial abundance as the health benefit endpoint.
科学证据越来越多地支持基于益生菌的食品补充剂对人类肠道健康的有益影响。本离体研究调查了三种基于益生菌的食品补充剂（分别含有 1）ES1、2）NCFM 和 3）NCFM、Lpc-37™、Bi-07™和 Bl-04™的组合）对肠道微生物群组成和代谢活性的影响。本研究使用来自六位健康供体的粪便样本，接种在结肠板系统上。在暴露于或不暴露于食品补充剂 48 小时后，测量了对整体微生物发酵（pH）、通过产生短链和支链脂肪酸（SCFA 和 BCAF）、氨、乳酸和微生物组成的微生物代谢活性变化的影响。联合配方益生菌对发酵过程的影响最大，其特征是丁酸产量显著增加，所有供体的 BCAF 和氨显著减少，双歧杆菌和乳杆菌生长显著刺激。我们的研究结果表明，联合配方益生菌显著影响健康个体的肠道微生物组，显示代谢活性和微生物丰度的变化作为健康获益终点。
