3‴--Foliamenthoyl-Rutin, a New Flavonoid Glycoside from the Roots of .

(Menyanthaceae) has been used as a medicinal herb in traditional medicines to treat conditions such as strangury, polyuria, swelling, and as a diuretic and antipyretic. In our ongoing research to discover novel structural and/or biological natural products in natural resources, five flavonoids, quercetin (), quercitrin (), isoquercetin (), quercetin-3--vicianoside (), and rutin (), as well as a new flavonoid glycoside, 3‴--foliamenthoyl-rutin (), were isolated from the MeOH extract of roots. The chemical structure of the new compound () was determined by analyzing 1D and 2D NMR spectra and high-resolution (HR) electrospray ionization mass spectroscopy (ESIMS), along with a chemical reaction. The wound-healing activities of the isolated compounds (-) were evaluated using a HaCaT cell scratch test. Among the isolates, isoquercetin (), quercetin-3--vicianoside (), and 3‴--foliamenthoyl-rutin () promoted HaCaT cell migration over scratch wounds, with compound being the most effective. Our findings provide experimental data supporting the potential of quercetin-3--vicianoside () as a wound-healing agent.