Universidad Autónoma de Sinaloa, Gral. Ángel Flores s/n, Apdo. Postal 80000, Culiacán, Sinaloa, Mexico.
Centro de Investigación en Alimentación y Desarrollo, A.C., CTAOV, Apdo. Postal 1735, Hermosillo, Sonora 83304, Mexico.
Food Chem. 2016 Aug 1;204:420-426. doi: 10.1016/j.foodchem.2016.02.130. Epub 2016 Feb 23.
Quercetin-3-O-rutinoside (rutin), quercetin-3-O-glucoside (isoquercetin) and quercetin have shown antioxidant, cytoprotective, vasoprotective, antiproliferative and antiinflammatory properties. The aim of this work was to determine the conversion of rutin to isoquercetin and quercetin during the production of poly(l-lactic acid) films with potential to deliver these flavonoids toward tissues, pharmaceuticals or food matrices. Three poly(l-lactic acid) formulations with 17.7, 39.6 and 39.1mg/g of rutin were prepared by the extrusion process. Processing temperatures (130-165°C) promoted the deglycosylation of rutin to produce isoquercetin and subsequently quercetin, identified by high performance liquid chromatography coupled to mass spectrometry. The effect of the process on the antioxidant activity of the films was determined by measuring the capacity to scavenge 2,2 diphenyl-1-picrylhydrazyl radicals. The material with the highest proportion of quercetin showed the highest antioxidant activity which could be used to produce delivering devices of the flavonoids to tissues, pharmaceuticals or food matrices.
槲皮素-3-O-芸香糖苷（芦丁）、槲皮素-3-O-葡萄糖苷（异槲皮苷）和槲皮素具有抗氧化、细胞保护、血管保护、抗增殖和抗炎作用。本工作的目的是确定在具有向组织、药物或食品基质递送这些类黄酮潜力的聚（L-乳酸）薄膜生产过程中，芦丁向异槲皮苷和槲皮素的转化。通过挤出工艺制备了三种含有 17.7、39.6 和 39.1mg/g 芦丁的聚（L-乳酸）配方。加工温度（130-165°C）促进了芦丁的去糖基化，生成异槲皮苷，随后通过高效液相色谱-质谱联用鉴定出芦丁。通过测量清除 2,2-二苯基-1-苦基肼自由基的能力来确定该过程对薄膜抗氧化活性的影响。具有最高比例槲皮素的材料显示出最高的抗氧化活性，可用于生产向组织、药物或食品基质递送类黄酮的给药装置。
