Pírek Pavlína, Kryštofová Karolína, Kováčová Ingrid, Kromerová Anna, Zachová Dagmar, Helia Ondřej, Panzarová Klára, Fajkus Jiří, Zdráhal Zbyněk, Lochmanová Gabriela, Fojtová Miloslava
Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, 62500 Brno, Czech Republic.
National Centre for Biomolecular Research, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic.
Plants (Basel). 2023 Dec 15;12(24):4177. doi: 10.3390/plants12244177.
The ability for plant regeneration from dedifferentiated cells opens up the possibility for molecular bioengineering to produce crops with desirable traits. Developmental and environmental signals that control cell totipotency are regulated by gene expression via dynamic chromatin remodeling. Using a mass spectrometry-based approach, we investigated epigenetic changes to the histone proteins during callus formation from roots and shoots of seedlings. Increased levels of the histone H3.3 variant were found to be the major and most prominent feature of 20-day calli, associated with chromatin relaxation. The methylation status in root- and shoot-derived calli reached the same level during long-term propagation, whereas differences in acetylation levels provided a long-lasting imprint of root and shoot origin. On the other hand, epigenetic signs of origin completely disappeared during 20 days of calli propagation in the presence of histone deacetylase inhibitors (HDACi), sodium butyrate, and trichostatin A. Each HDACi affected the state of post-translational histone modifications in a specific manner; NaB-treated calli were epigenetically more similar to root-derived calli, and TSA-treated calli resembled shoot-derived calli.
从去分化细胞再生植株的能力为分子生物工程培育具有理想性状的作物开辟了可能性。控制细胞全能性的发育和环境信号通过动态染色质重塑由基因表达调控。我们采用基于质谱的方法，研究了幼苗根和芽形成愈伤组织过程中组蛋白的表观遗传变化。发现组蛋白H3.3变体水平升高是20天龄愈伤组织的主要且最显著特征，与染色质松弛相关。在长期继代培养过程中，根和芽来源的愈伤组织中的甲基化状态达到相同水平，而乙酰化水平的差异则提供了根和芽来源的持久印记。另一方面，在存在组蛋白去乙酰化酶抑制剂（HDACi）、丁酸钠和曲古抑菌素A的情况下，愈伤组织培养20天期间，起源的表观遗传特征完全消失。每种HDACi以特定方式影响组蛋白翻译后修饰状态；经丁酸钠处理的愈伤组织在表观遗传上更类似于根来源的愈伤组织，经曲古抑菌素A处理的愈伤组织类似于芽来源的愈伤组织。
