Microbiology and Cell Science Department, IFAS, University of Florida, Gainesville, FL 32611, USA.
Molecular Genetics and Microbiology Department, College of Medicine, University of Florida, Gainesville, FL 32611, USA.
Viruses. 2023 Dec 16;15(12):2443. doi: 10.3390/v15122443.
Norovirus infection is influenced by the presence of commensal bacteria, and both human and murine norovirus (MNV) bind to these bacteria. These virus-bacterial interactions, as well as MNV infection, promote the increased production of bacterial extracellular vesicles (bEVs). However, no correlation has been made between specific bacterial groups, their vesicles, and their impact on norovirus infection. The current study evaluated the impact of select bacterial compositions of murine microbiomes using antibiotic (ABX) cocktails on MNV infection and bEV production. The goal of this research was to determine if increases in bEVs following MNV infection in mice were associated with changes in specific bacterial populations. Bacterial taxa were found to be differentially abundant in both ABX-treated and untreated mice, with the greatest change in bacterial taxa seen in mice treated with a broad-spectrum ABX cocktail. Specifically, were found to be differentially abundant between a variety of treatment factors, including MNV infection. Overall, these results demonstrate that MNV infection can alter the abundance of bacterial taxa within the microbiota, as well as their production of extracellular vesicles, and that the use of selective antibiotic treatments can allow the detection of viral impacts on the microbiome that might otherwise be masked.
诺如病毒感染受共生细菌的存在影响，人类和鼠诺如病毒 (MNV) 均与这些细菌结合。这些病毒-细菌相互作用以及 MNV 感染会促进细菌细胞外囊泡 (bEV) 的大量产生。然而，尚未确定特定细菌群及其囊泡与诺如病毒感染之间的相关性。本研究使用抗生素 (ABX) 鸡尾酒评估了选择的鼠微生物组细菌组成对 MNV 感染和 bEV 产生的影响。本研究的目的是确定 MNV 感染后小鼠 bEV 增加是否与特定细菌种群的变化有关。在 ABX 处理和未处理的小鼠中均发现细菌分类群存在差异丰度，在接受广谱 ABX 鸡尾酒治疗的小鼠中观察到细菌分类群的变化最大。具体而言，在包括 MNV 感染在内的各种处理因素之间发现存在差异丰度。总体而言，这些结果表明 MNV 感染可以改变微生物组内细菌分类群的丰度及其细胞外囊泡的产生，并且选择性抗生素处理的使用可以检测到病毒对微生物组的影响，否则这些影响可能会被掩盖。
