Microbiology and Cell Science Department, IFAS, University of Florida, Gainesville, FL 32611, USA.
Viruses. 2020 Jul 14;12(7):759. doi: 10.3390/v12070759.
The presence of commensal bacteria enhances both acute and persistent infection of murine noroviruses. For several enteric viral pathogens, mechanisms by which these bacteria enhance infection involve direct interactions between the virus and bacteria. While it has been demonstrated that human noroviruses bind to a variety of commensal bacteria, it is not known if this is also true for murine noroviruses. The goal of this study was to characterize interactions between murine noroviruses and commensal bacteria and determine the impact of bacterial growth conditions, incubation temperature and time, on murine norovirus attachment to microbes that comprise the mammalian microbiome. We show that murine noroviruses bind directly to commensal bacteria and show similar patterns of attachment as human norovirus VLPs examined under the same conditions. Furthermore, while binding levels are not impacted by the growth phase of the bacteria, they do change with time and incubation temperature. We also found that murine norovirus can bind to a commensal fungal species, .
共生菌的存在增强了鼠诺如病毒的急性和持续性感染。对于几种肠道病毒病原体，这些细菌增强感染的机制涉及病毒和细菌之间的直接相互作用。虽然已经证明人类诺如病毒与多种共生菌结合，但鼠诺如病毒是否也是如此尚不清楚。本研究的目的是描述鼠诺如病毒与共生菌之间的相互作用，并确定细菌生长条件、孵育温度和时间对构成哺乳动物微生物组的微生物附着到鼠诺如病毒的影响。我们表明，鼠诺如病毒直接与共生菌结合，并显示出与在相同条件下检查的人类诺如病毒 VLPs 相似的附着模式。此外，尽管结合水平不受细菌生长阶段的影响，但它们确实会随时间和孵育温度而变化。我们还发现鼠诺如病毒可以与一种共生真菌物种 结合。
