Facultad de Ciencias de la Salud, Universidad San Jorge. Campus Universitario, Autov A23 km 299, 50830 Villanueva de Gállego, Zaragoza, Spain.
Faculty of Health and Sports Sciences, University of Zaragoza, Campus of Huesca, 22002 Huesca, Spain.
ACS Biomater Sci Eng. 2024 Jan 8;10(1):178-190. doi: 10.1021/acsbiomaterials.3c00859. Epub 2023 Dec 23.
Cryopreservation of cells, tissues, and organs is widely used in the biomedical and research world. There are different cryopreservatives that are used for this process; however, many of them, such as DMSO, are used despite the problems they present, mainly due to the toxicity it presents to certain types of samples. The aim of this Review is to highlight the different types of substances used in the cryopreservation process. It has been shown that some of these substances are well-known, as in the case of the families of alcohols, sugars, sulfoxides, etc. However, in recent years, other compounds have appeared, such as ionic liquids, deep eutectic solvents, or certain polymers, which open the door to new cryopreservation methods and are also less toxic to frozen samples.
细胞、组织和器官的冷冻保存广泛应用于生物医学和研究领域。有不同的冷冻保存剂用于这个过程；然而，其中许多冷冻保存剂，如 DMSO，尽管存在问题仍在使用，主要是由于其对某些类型的样本存在毒性。本综述的目的是强调冷冻保存过程中使用的不同类型的物质。已经表明，其中一些物质是众所周知的，如醇、糖、亚砜等家族。然而，近年来，出现了其他化合物，如离子液体、深共晶溶剂或某些聚合物，为新的冷冻保存方法开辟了道路，对冷冻样本的毒性也较小。
