School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, Australia.
School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, Australia.
J Colloid Interface Sci. 2021 Dec;603:491-500. doi: 10.1016/j.jcis.2021.06.096. Epub 2021 Jun 17.
Cryopreservation has facilitated considerable advances in both medical technology and scientific research. However, further developments have been limited by the relatively low number of effective cryoprotective agents. Even after fifty years of research, most protocols rely on the same two toxic agents, i.e. dimethylsulfoxide or glycerol. Ionic liquids are a class of promising solvents which are known glass formers and may offer a less-toxic alternative. The research presented here investigates ten protic ionic liquids as potential cryoprotective agents. The liquids are screened for key properties including cellular toxicity, permeability and thermal behaviour. The most promising, ethylammonium acetate, was then tested as a cryoprotective agent on a model cell line and was found to be as effective as the common cryoprotectant, dimethylsulfoxide. This work reports the first use of a protic ionic liquid as an effective cryoprotective agent for a mammalian cell line. This will inform the development of a suite of potential new ionic liquid-based cryoprotectants that could potentially allow the cryopreservation of new cell types.
冷冻保存极大地促进了医学技术和科学研究的发展。然而，进一步的发展受到有效冷冻保护剂数量相对较少的限制。即使经过五十年的研究，大多数方案仍然依赖于两种有毒的试剂，即二甲基亚砜或甘油。离子液体是一类有前途的溶剂，它们是已知的玻璃形成剂，可能提供毒性较小的替代品。本研究介绍了十种质子离子液体作为潜在的冷冻保护剂。对这些液体的关键性质进行了筛选，包括细胞毒性、渗透性和热行为。最有前途的是乙酸乙酯铵，然后在模型细胞系上作为冷冻保护剂进行测试，发现它与常用的冷冻保护剂二甲基亚砜一样有效。这项工作首次报道了质子离子液体作为一种有效的哺乳动物细胞系冷冻保护剂的使用。这将为一系列潜在的新型离子液体基冷冻保护剂的开发提供信息，这些冷冻保护剂可能允许新细胞类型的冷冻保存。
