National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Collage of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Bioresour Technol. 2024 Feb;394:130231. doi: 10.1016/j.biortech.2023.130231. Epub 2023 Dec 22.
In this study, a surfactant-assisted diluted ethylenediamine (EDA) fractionation process was investigated for co-generation of technical lignin and biobutanol from corn stover. The results showed that the addition of PEG 8000 significantly enhanced cellulose recovery (88.9 %) and lignin removal (68.9 %) in the solid fraction. Moreover, the pulp achieved 86.5 % glucose yield and 82.6 % xylose yield in enzymatic hydrolysis. Structural characterization confirmed that the fractionation process promoted the preservation of active β-O-4 bonds (35.8/100R) in isolated lignin and functionalized the lignin through structural modification using EDA and surfactant grafting. The enzymatic hydrolysate of the pulps yielded a sugar solution for acetone-butanol-ethanol (ABE) fermentation, resulting in an ABE concentration of 15.4 g/L and an overall yield of 137.2 g/Kg of dried corn stalk. Thus, the surfactant-assisted diluted EDA fractionation has the potential to enhance the overall economic feasibility of second-generation biofuels production within the framework of biorefinery.
在这项研究中，研究了一种表面活性剂辅助的稀释乙二胺（EDA）分级工艺，以从玉米秸秆中共同生产技术木质素和生物丁醇。结果表明，PEG8000 的添加显著提高了固体部分中的纤维素回收率（88.9%）和木质素去除率（68.9%）。此外，纸浆在酶水解中实现了 86.5%的葡萄糖产率和 82.6%的木糖产率。结构表征证实，分级过程通过 EDA 和表面活性剂接枝的结构修饰促进了活性β-O-4 键（35.8/100R）在分离木质素中的保留，并对木质素进行了功能化。纸浆的酶解产物得到了用于丙酮丁醇乙醇（ABE）发酵的糖溶液，得到了 15.4g/L 的 ABE 浓度和 137.2g/Kg 干玉米秸秆的总产率。因此，在生物炼制框架内，表面活性剂辅助的稀释 EDA 分级法有可能提高第二代生物燃料生产的整体经济可行性。
