稀堿预处理对玉米秸秆不同部位转化为可发酵糖的影响及其在丙酮丁醇乙醇发酵中的应用。
Effect of dilute alkaline pretreatment on the conversion of different parts of corn stalk to fermentable sugars and its application in acetone-butanol-ethanol fermentation.
机构信息
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
出版信息
Bioresour Technol. 2016 Jul;211:117-24. doi: 10.1016/j.biortech.2016.03.076. Epub 2016 Mar 15.
To investigate the effect of dilute alkaline pretreatment on different parts of biomass, corn stalk was separated into flower, leaf, cob, husk and stem, which were treated by NaOH in range of temperature and chemical loading. The NaOH-pretreated solid was then enzymatic hydrolysis and used as the substrate for batch acetone-butanol-ethanol (ABE) fermentation. The results demonstrated the five parts of corn stalk could be used as potential feedstock separately, with vivid performances in solvents production. Under the optimized conditions towards high product titer, 7.5g/L, 7.6g/L, 9.4g/L, 7g/L and 7.6g/L of butanol was obtained in the fermentation broth of flower, leaf, cob, husk and stem hydrolysate, respectively. Under the optimized conditions towards high product yield, 143.7g/kg, 126.3g/kg, 169.1g/kg, 107.7g/kg and 116.4g/kg of ABE solvent were generated, respectively.
为了研究稀堿预处理对生物质不同部位的影响，将玉米秸秆分为花、叶、穗、壳和茎，分别用 NaOH 在一定的温度和化学负荷下进行处理。然后将 NaOH 预处理后的固体进行酶解，并用作分批丙酮-丁醇-乙醇（ABE）发酵的底物。结果表明，玉米秸秆的五个部分可以分别作为潜在的原料，在溶剂生产方面表现出色。在高产物浓度的优化条件下，从花、叶、穗、壳和茎水解液发酵液中分别获得 7.5g/L、7.6g/L、9.4g/L、7g/L 和 7.6g/L 的丁醇。在高产物产率的优化条件下，分别生成 143.7g/kg、126.3g/kg、169.1g/kg、107.7g/kg 和 116.4g/kg 的 ABE 溶剂。
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