Steven F S, Jackson H, Jackson N C, Wong T L
Br J Cancer. 1987 Jan;55(1):29-32. doi: 10.1038/bjc.1987.6.
Fluorescence probes for the active centre of an enzyme associated with tumour cells have been used to locate leukaemia cells in a model rat system. These fluorescent techniques are inexpensive and rapid to carry out. The leukaemic cells can be located by fluorescence microscopy in frozen sections, wax embedded sections and resin embedded sections. The technique is illustrated with reference to sections of leukaemic rat kidney, epididymis and testis. These studies confirm earlier histological findings employing conventional staining techniques and have the advantage that individual leukaemia cells can be detected in leukaemic animals undergoing drug therapy. The evidence suggests that these techniques will be of value in further studies of the design of drugs directed to leukaemia cells.
用于肿瘤细胞相关酶活性中心的荧光探针已被用于在模型大鼠系统中定位白血病细胞。这些荧光技术成本低廉且操作快速。白血病细胞可通过荧光显微镜在冰冻切片、石蜡包埋切片和树脂包埋切片中定位。以白血病大鼠肾脏、附睾和睾丸的切片为例对该技术进行了说明。这些研究证实了早期采用传统染色技术的组织学发现，并且具有能够在接受药物治疗的白血病动物中检测到单个白血病细胞的优势。有证据表明，这些技术将在针对白血病细胞的药物设计的进一步研究中具有价值。
