Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China; Department of Laboratory Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China; Gene Diagnostic Research Center, the First Affiliated Hospital, Fujian Medical University, Fuzhou, China; Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China; Fujian Clinical Research Center for Laboratory Medicine of Immunology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China; Department of Laboratory Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China; Gene Diagnostic Research Center, the First Affiliated Hospital, Fujian Medical University, Fuzhou, China; Fujian Key Laboratory of Laboratory Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China; Fujian Clinical Research Center for Laboratory Medicine of Immunology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.
Clin Chim Acta. 2024 Jan 15;553:117739. doi: 10.1016/j.cca.2023.117739. Epub 2023 Dec 23.
The significance of large (LHB) and middle (MHB) HBV surface proteins in chronic hepatitis B (CHB) remains uncertain. This study investigates the role of LHB and MHB in different infection phases and liver diseases.
Serum samples from 217 patients with HBV chronic infection, CHB, liver cirrhosis (LC), and hepatocellular carcinoma (HCC) were subjected to quantification of LHB and MHB using ELISA.
Positive correlations were observed among LHB, MHB, and LHB/HBsAg, with HBV serum markers including HBsAg, HBeAg, and HBV DNA. (P < 0.0001). In HBeAg-positive chronic infection, LHB and MHB were higher than in HBeAg-positive CHB (P < 0.01). In HBeAg-negative chronic infection, LHB and MHB were lower than in HBeAg-negative CHB (P < 0.01). ROC analysis identified LHB and MHB as potential discriminators of CHB and chronic infection. LC and HCC exhibited lower LHB, MHB, and MHB/HBsAg than CHB (P < 0.05). Multivariate analysis found that age and the MHB/HBsAg serve as independent factors for the progression of CHB to end stage of liver disease.
LHB and MHB emerge as novel biomarkers distinguishing chronic infection and CHB. MHB/HBsAg shows promise as a predictor for CHB progression.
大（LHB）和中（MHB）HBV 表面蛋白在慢性乙型肝炎（CHB）中的意义仍不确定。本研究旨在探讨 LHB 和 MHB 在不同感染阶段和肝脏疾病中的作用。
采用 ELISA 法检测 217 例 HBV 慢性感染者、CHB、肝硬化（LC）和肝细胞癌（HCC）患者血清中 LHB 和 MHB 的含量。
LHB、MHB 与 LHB/HBsAg 呈正相关，与 HBV 血清标志物 HBsAg、HBeAg 和 HBV DNA 呈正相关（P<0.0001）。在 HBeAg 阳性慢性感染中，LHB 和 MHB 均高于 HBeAg 阳性 CHB（P<0.01）。在 HBeAg 阴性慢性感染中，LHB 和 MHB 均低于 HBeAg 阴性 CHB（P<0.01）。ROC 分析表明，LHB 和 MHB 可作为 CHB 和慢性感染的潜在鉴别标志物。LC 和 HCC 患者的 LHB、MHB 和 MHB/HBsAg 水平均低于 CHB 患者（P<0.05）。多因素分析发现，年龄和 MHB/HBsAg 是 CHB 进展为终末期肝病的独立因素。
LHB 和 MHB 可作为鉴别慢性感染和 CHB 的新型生物标志物。MHB/HBsAg 有望成为 CHB 进展的预测指标。
