Thakur Geetika, Sutaoney Priya, Joshi Veenu, Ghosh Prabir
Center for Basic Science, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh 492010 India.
Present Address: Department of Microbiology, Kalinga University, Raipur, Chhattisgarh 492101 India.
3 Biotech. 2024 Jan;14(1):21. doi: 10.1007/s13205-023-03860-0. Epub 2023 Dec 23.
The expense of cellulase enzymes is the main barrier to the enzymatic saccharification of biomass. Numerous tactics, such as the utilizing inexpensive lignocellulosic substrates as well as economically feasible fermentation techniques for the production of the enzyme may reduce the cost of cellulases. The present investigation was aimed to improve cellulase production employing potential cellulolytic soil fungi, NFCCI 5299 using wheat bran as substrate. Employing response surface methodology (RSM) with central composite design (CCD), the most efficient process parameters were determined. The ideal conditions for the synthesis of carboxy methyl cellulase (CMCase) and filter paper cellulase activity (FPase) were 6 days of incubation, inoculum size of 4 mycelial disc, 125 rpm of agitation, and 3.5% of wheat bran. The significant mycelial development and enzymatic digestion of wheat bran were discovered by scanning electron microscopy (SEM) and fourier transform infrared (FTIR) analysis. The findings suggested that it can be practicable to use wheat bran as substrate under submerged fermentation utilizing NFCCI 5299 for efficient cellulase production.
纤维素酶的成本是生物质酶促糖化的主要障碍。许多策略，如使用廉价的木质纤维素底物以及经济可行的酶生产发酵技术，可能会降低纤维素酶的成本。本研究旨在利用潜在的纤维素分解土壤真菌NFCCI 5299，以麦麸为底物提高纤维素酶的产量。采用响应面法（RSM）和中心复合设计（CCD），确定了最有效的工艺参数。合成羧甲基纤维素酶（CMCase）和滤纸纤维素酶活性（FPase）的理想条件是培养6天、接种量为4个菌丝盘、搅拌速度为125 rpm以及麦麸含量为3.5%。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）分析发现了显著的菌丝生长和麦麸的酶解作用。研究结果表明，利用NFCCI 5299在深层发酵条件下以麦麸为底物高效生产纤维素酶是可行的。
