一种用于定量血液粒细胞吞噬能力的灵敏方法。

A sensitive procedure for quantifying the phagocytic competence of blood granulocytes.

作者信息

Spangenberg P, Crawford N

出版信息

Biosci Rep. 1986 Aug;6(8):715-20. doi: 10.1007/BF01116538.

Abstract

The phagocytic activity of blood granulocytes can be quantitatively assayed by ingestion of opsonised paraffin oil droplets containing the dye "Oil Red-O" (Stossel, T.P., Mason, R.J., Hartwig, J., and Vaughan, M. (1972) J. Clin. Invest. 51: 615-624). We have modified this assay by incorporating [3H]glycerol into the oil droplets which allows a more sensitive and reproducible measurement of the phagocytic competence of blood granulocytes even at very low cell counts. Comparative studies after one day storage of the blood at 4 degrees C is feasible since they retain 84% of the phagocytic capacity measured when isolated from fresh blood.

摘要

血液粒细胞的吞噬活性可通过摄取含有染料“油红O”的调理石蜡油滴进行定量测定（斯托塞尔，T.P.，梅森，R.J.，哈特维希，J.，和沃恩，M.（1972年）《临床研究杂志》51: 615 - 624）。我们对该测定方法进行了改进，将[3H]甘油掺入油滴中，这样即使在细胞计数非常低的情况下，也能更灵敏且可重复地测量血液粒细胞的吞噬能力。在4℃下储存一天后的血液进行比较研究是可行的，因为它们保留了从新鲜血液中分离时所测吞噬能力的84%。